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Purified anti-Fyn Antibody

Pricing & Availability
Clone
FYN-59 (See other available formats)
Regulatory Status
RUO
Other Names
Tyrosine kinase p59fyn, Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-protein kinase fyn
Isotype
Mouse IgG
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Product Citations
publications
FYN-59_PURE_Fyn_Antibody_WB_050118
Total lysates (15 µg protein) from RPMI8226 (low expression, negative control), Jurkat, U87-MG and EL-4 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-Fyn antibody, clone FYN-59 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053, Cat. No. 405306) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M is the MW ladder.
  • FYN-59_PURE_Fyn_Antibody_WB_050118
    Total lysates (15 µg protein) from RPMI8226 (low expression, negative control), Jurkat, U87-MG and EL-4 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-Fyn antibody, clone FYN-59 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053, Cat. No. 405306) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M is the MW ladder.
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626501 25 µg £77
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626502 100 µg £182
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Description

Fyn is a 59 kD protein tyrosine kinase and a member of the src subfamily. There are two reported isoforms of Fyn; FynT found in T cells, B cells and thymocytes, and FynB found in neuronal cells. Fyn has been shown to control cell growth, interact with the p85 subunit of PI3K, control high affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation. Fyn is known to be modified by phosphorylation and palmitoylation and has been shown to interact with Fyn binding protein, p85 PI3K, high affinity IgE receptor, and NCAM140. The FYN-59 monoclonal antibody recognizes human and mouse Fyn and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human Fyn (aa 7-176) expressed in E coli
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store antibody solution undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Laird RM, et al. 2010. J. Immunol. 185:6518. PubMed
Product Citations
  1. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  2. Doi H, et al. 2014. Clin Immunol. 184:150. PubMed
  3. Nelson MH, et al. 2015. J Immunol. 1737:194. PubMed
  4. Shi W, et al. 2020. J Biol Chem. 295:7653. PubMed
  5. Dwyer A, et al. 2016. J Leukoc Biol. 100: 163 - 175. PubMed
  6. Laird R, et al. 2010. J Immunol. 185:6518. PubMed
  7. Laird R, Hayes S 2010. PLoS One. 5:e8899. PubMed
  8. Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed
  9. Bland T, et al. 2019. Endocrinology. 161(2):. PubMed
RRID
AB_2278824 (BioLegend Cat. No. 626501)
AB_2278824 (BioLegend Cat. No. 626502)

Antigen Details

Structure
Protein tyrosine kinase family, src subfamily. Isoforms fynT, fynB, 59 kD
Distribution

FynT T cells, thymocytes, B cells; fynB neuronal cells

Function
Controls cell growth, interacts with p85 subunit of PI3K. Involved in high affinity IgE receptor-mediated mast cell degranulation
Interaction
Fyn binding protein, p85 PI3K, high affinity IgE receptor, NCAM140
Modification
Phosphorylation, Palmitoylation
Cell Type
T cells, Thymocytes, B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Goldsmith J, et al. 2002. Biochem. Biophys. Res. Comm. 298:501.
2. Prasad K, et al. 1993. P. Natl. Acad. Sci. USA 90:7366.
3. Kawakami T, et al. 1986. Mol. Cell. Biol. 6:4195.

Regulation
Activated by TCR engagement or anti-CD3
Gene ID
2534 View all products for this Gene ID
UniProt
View information about Fyn on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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