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Purified anti-GABRA2 Antibody

Pricing & Availability
Clone
N399/19 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-Aminobutyric Acid Type A Receptor Subunit Alpha2, GABRA2, GABA(A) receptor subunit alpha-2, EIEE78, DEE78
Isotype
Mouse IgG1, κ
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Product Citations
publications
N399slash19_PURE_GABRA2_Antibody_102021
IHC staining of anti-GABRA2 antibody (clone N399/19) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
  • N399slash19_PURE_GABRA2_Antibody_102021
    IHC staining of anti-GABRA2 antibody (clone N399/19) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
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601552 100 µg £113
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Description

GABRA2 is a type of alpha subunit which comprises two of the five subunits of the pentameric GABA-A ionotropic receptor. In response to GABA (gamma-aminobutyric acid) binding, the GABA-A receptor permits the passage of negatively-charged chloride ions, inhibiting action potential firing. GABRA2 is present in approximately 35% of all GABA-A receptors and is expressed primarily in the hippocampus and forebrain, where it plays a role in anxiolytic activity. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cytoplasmic domain of recombinant rat GABA(A) Receptor Subunit Alpha-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F - Reported in the Literature
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Scott H, et al. 2020. Neurobiol. Stress. 13:100281. 
RRID
AB_2904444 (BioLegend Cat. No. 601552)

Antigen Details

Structure
GABRA2 is a 451 amino acid protein with a predicted molecular weight of 51.3 kD.
Distribution

GABRA2 is expressed in GABA-ergic neurons of the central and peripheral nervous system.

Function
GABRA2 is a ligand-gated ion channel for chloride (Cl-) and to a lesser extent bicarbonate (HCO3-) ions.
Ligand/Receptor
GABRA2 is a receptor for GABA (γ-aminobutyric acid)
Antigen References
  1. Sigel E, et al. 2012. J Biol. Chem. 287(48):40224-40231
Gene ID
2555 View all products for this Gene ID
UniProt
View information about GABRA2 on UniProt.org
Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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