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Purified anti-Gasdermin-D (N-terminus) Antibody

Pricing & Availability
Clone
W18029B (See other available formats)
Regulatory Status
RUO
Other Names
GSDMD, DF5L, DFNA5L, GSDMC1,FKSG10, Gadermin domain-containing protein 1
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W18029A_PURE_GasderminD_Antibody_092220
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Gasdermin-D antibody (clone W18029B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18029A_PURE_GasderminD_Antibody_092220
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Gasdermin-D antibody (clone W18029B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18029A_PURE_GasderminD_Antibody_2_092220
    Whole cell extracts (250 µg total protein) prepared from PC-3 cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, λ isotype ctrl antibody (Cat. No. 403801) or purified anti-Gasdermin-D antibody (clone W18029B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rat control antibody against a separate epitope of Gasdermin-D. Lane M: Molecular weight marker.
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939701 25 µg £81
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939702 100 µg £201
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Description

Gasdermin D (N-terminus) is a member of the Gasdermin family of pore-forming proteins involved in pyroptosis. Gasdermin-D is vital to inducing pyroptotic cell death in response to pathogen and damage-associated molecular patterns, thus performing a role crucial to innate immune defense. Gasdermin-D is localized to the cytosol in an inactive state but, following cleavage into N and C-terminal fragments by inflammatory caspases Caspase-1, 4, or 5, the N-terminal fragment translocates to the plasma membrane where it forms large oligomeric pores. Pore-formation results in a disruption in the cell’s osmotic potential and subsequent cell lysis. Secretion of pro-inflammatory cytokines IL-1β and IL-18 from bone marrow dendritic cells (BMDCs), bone marrow derived macrophages (BMDMs), and neutrophils is also dependent upon Gasdermin-D, both through release by cell lysis and by direct passage through pores formed by its N-terminal fragment in the plasma membrane. Gasdermin-D exhibits bactericidal properties through the binding of its N-terminus to the cardiolipin of bacterial cell membranes and subsequent membrane perforation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Gasdermin-D corresponding to the N-terminus of the protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting and immunoprecipitation the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using NTERA-2 (negative control) and PC-3 (positive control) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in weak staining with high background.

W18029B is predicted to recognize the pro-pyroptotic N-terminal fragment of cleaved Gasdermin-D. The immunogen used to generate this clone is fully contained within the sequence of the N-terminal fragment.

RRID
AB_2888893 (BioLegend Cat. No. 939701)
AB_2888893 (BioLegend Cat. No. 939702)

Antigen Details

Structure
Gasdermin-D is a 484 amino acid protein with an expected molecular weight of 53 kD.
Distribution

Ubiquitously expressed/Cytosol and plasma membrane

Function
Pyroptosis, inflammatory response
Biology Area
Innate Immunity, Mitochondrial Function
Molecular Family
Innate Immune Signaling
Antigen References

1. Heilig R, et al. 2017. Eur. J. Immunol. 48:584.
2. Kambara H, et al. 2018. Cell Reports. 22:2924.
3. Mulvihill E, et al. 2018. EMBO J. 37:98321
4. Shi J, et al. 2015. Nature. 526:660.

Gene ID
79792 View all products for this Gene ID
UniProt
View information about Gasdermin-D on UniProt.org
Go To Top Version: 1    Revision Date: 09/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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