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Purified anti-GATA4 Antibody

Pricing & Availability
Clone
W19078B (See other available formats)
Regulatory Status
RUO
Other Names
GATA-binding factor 4, GATA-4, TACHD, ASD2, VSD1, TOF
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19078B_Purified_GATA4_Antibody_030922
Hep G2 cells (filled histogram, positive control) and HeLa cells (open histogram, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.5 µg/test of purified anti-GATA4 (clone W19078B) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W19078B_Purified_GATA4_Antibody_030922
    Hep G2 cells (filled histogram, positive control) and HeLa cells (open histogram, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.5 µg/test of purified anti-GATA4 (clone W19078B) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W19078B_Purified_GATA4_Antibody_2_030922.
    Whole cell extracts (250 µg total protein) prepared from SH-SY5Y cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2a, κ isotype control (Cat. No. 400502) or purified anti-GATA4 (clone W19078B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of GATA4. Lane M: Molecular weight marker
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948601 25 µg £81
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Description

GATA binding protein 4 (GATA4) is a zinc-finger transcription factor that plays a crucial role in transcriptional activation and cell growth, differentiation, survival, and senescence. It is essential to cardiac development and function. Along with GATA6, it modulates cardiac stress response and regulates cardiomyocyte formation, replication, and function. GATA4 participates in second heart field transcription and differentiation and ventricular morphogenesis through downstream regulation of Mef2c and Hand2. Mutations in GATA4 can result in myocardial abnormalities, myocardial hypoplasia, absence of a proepicardium, abnormal valve formation, and endocardial cushion defects. In the intestine, GATA4, along with GATA6, is also responsible for regulating epithelial cytodifferentiation and structure, and for maintaining balance between enterocytes and secretory cells through the coordination of Notch signaling. GATA4 plays a vital role in human testicular development through its regulation of the expression of steroidogenic factors StAR, INHA, HSD3B2, and CYP19A1 and sex-determining genes SRY, AMH, and SOX9. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human GATA4 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells and SH-SY5Y cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in specific, but weak GATA4 staining. We therefore do not recommend this clone for the application.

While this clone detected GATA4 by WB, it also showed a very weak affinity for the protein. Because of this, we do not recommend this clone for the application.

This clone failed IHC testing due to lack of specificity for GATA4. We therefore do not recommend this clone for the application.

This clone is not compatible with our True Nuclear™ Transcription Factor and Cyto-Fast™ buffer sets. We recommend using using True-Phos™ Perm Buffer (Cat. No. 425401) for ICFC.

RRID
AB_2922649 (BioLegend Cat. No. 948601)

Antigen Details

Structure
GATA4 is a 442 amino acid protein with a predicted molecular weight of 44 kD
Distribution

Expressed in the heart, intestine, liver, endoderm, gonads/ Nucleus

Function
Transcription factor
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Lourenco D, et al. 2011. Proc Natl Acad Sci. USA. 4:1597-602.
  2. Walker E, et al. 2014. Dev Biol. 392:283-94.
  3. Zhou P, et al. 2012. Curr Top Dev Biol. 100:143-69.
Gene ID
2626 View all products for this Gene ID
UniProt
View information about GATA4 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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