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Purified anti-Gelsolin Antibody

Pricing & Availability
Clone
CPTC-Gelsolin-2 (See other available formats)
Regulatory Status
RUO
Other Names
GSN, AGEL, Actin-depolymerizing factor, ADF, Brevin, Epididymis Secretory Sperm Binding Protein, Amyloidosis, Finnish Type
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_1_062719.png
Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_1_062719.png
    Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_2_062719.png
    Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_3_062719.png
    IHC staining of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2) on formalin-fixed paraffin-embedded glioblastoma brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_4_062719.png
    IHC staining of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_5_100319
    ICC staining of purified anti-Gelsolin antibody (CPTC-Gelsolin-2) on Drosophila S2 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation wit 2.5 µg/ml of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 10 µm
Cat # Size Price Quantity Check Availability Save
866501 25 µg £73
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866502 100 µg £189
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Description

After severing, gelsolin remains bound to the barbed end of the actin filament, preventing the reannealing of severed filaments. Gelsolin is crucial for the maintenance of actin homeostasis. Cells from gelsolin null mice exhibit a variety of defects, such as pronounced stress fibers formation, defective chemotaxis, and wound healing. Dysfunction of Gelsolin has been indicated in multiple diseases include cancer, infection, inflammation, cardiac injury, pulmonary disease, and neurodegeneration. Gelsolin is proteolytically cleaved and rendered inactive in Alzheimer’s disease (AD). Overexpression of gelsolin significantly reduces amyloid beta load in brains of AD transgenic mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length gelsolin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810787 (BioLegend Cat. No. 866501)
AB_2810787 (BioLegend Cat. No. 866502)

Antigen Details

Structure
Human gelsolin is a 782 amino acid protein with a molecular mass of 86 kD.
Distribution

Tissue distribution: Central nervous system, phagocytic cells, platelets, fibroblasts, non-muscle cells, smooth and skeletal muscle cells.
Cellular distribution: Cytoskeleton and extracellular

Function
Gelsolin is a calcium-binding, actin-modulating protein. It can promote the assembly of actin monomers into filaments as well as sever actin filaments.
Interaction
Interacts with actin and fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X.
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroscience
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Ji L, et al. 2015. J Alzheimers Dis. 44(1):13-25
  2. Asch HL, et al. 1996. Cancer Res. 56(21):4841-5.
  3. Sun HQ, et al. 1999. J Biol Chem. 274(47):33179-82.
  4. Ji L, et al. 2009. J Alzheimers Dis. 18(1):105-11.
Gene ID
2934 View all products for this Gene ID
UniProt
View information about Gelsolin on UniProt.org

Other Formats

View All Gelsolin Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Gelsolin CPTC-Gelsolin-2 WB,IHC-P,ICC
Biotin anti-Gelsolin CPTC-Gelsolin-2 ICC
Go To Top Version: 2    Revision Date: 10/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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