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Purified anti-Gemin 6 Antibody

Pricing & Availability
Clone
6H5/Gemin6 (See other available formats)
Regulatory Status
RUO
Other Names
Gemin6
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H5slashGemin6_Purified_Gemin-6_Antibody_042919.png
Western blot of purified anti-Gemin 6 antibody (clone 6H5/Gemin6). Lane 1: Molecular weight marker; Lane 2: 40µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 10 µg of HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 6H5slashGemin6_Purified_Gemin-6_Antibody_042919.png
    Western blot of purified anti-Gemin 6 antibody (clone 6H5/Gemin6). Lane 1: Molecular weight marker; Lane 2: 40µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 10 µg of HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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862301 25 µg £73
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862302 100 µg £189
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Description

Gem-associated protein 6 (Gemin 6) is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells.  Gemin 6 has been shown to form heterodimers with Gemin 7, and together they bind to unrip and sequester the SMN complex in the cytoplasm for small nuclear ribonucleoproteins (snRNP) assembly3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is more reactive to mouse compared to human or rat Gemin 6. The recommended usage concentration for Western blotting in mouse is 0.2 - 1 µg/ml, and  in human and rat 5.0 - 10 µg/mL

Application References

(PubMed link indicates BioLegend citation)
  1. Massenet S, et al. 2002. Mol Cell Biol. 22(18):6533 (WB)
  2. Battle DJ, et al. 2007. J Biol Chem. 282(38):27953 (IP)
Product Citations
  1. Bizen N, et al. 2022. Cell Death Differ. . PubMed
RRID
AB_2810749 (BioLegend Cat. No. 862301)
AB_2810749 (BioLegend Cat. No. 862302)

Antigen Details

Structure
Gemin 6 is a 167 amino acid protein with an apparent molecular mass of ~19 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and cytosol

Function
Gemin 6 is a component of the SMN complex essential for the formation of snRNP complexes in Eukaryotic cells.
Interaction
Gemin-7, unrip
Cell Type
Mature Neurons, Neurons
Biology Area
Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience
Molecular Family
Nuclear Markers
Antigen References
  1. Lanfranco M, et al. 2017. FEBS Lett. 591(21):3600
  2. Massenet S, et al. 2002. Mol Cell Biol. 22(18):6533
  3. Battle DJ, et al. 2007. J Biol Chem. 282(38):27953
Gene ID
79833 View all products for this Gene ID
UniProt
View information about Gemin 6 on UniProt.org
Go To Top Version: 2    Revision Date: 06/14/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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