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Purified anti-GFAP Antibody

Pricing & Availability
Clone
MCA-5C10 (See other available formats)
Regulatory Status
RUO
Other Names
GFAP, Glial fibrillary acidic protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_MCA-5C10_Purified_GFAP_Antibody_2_050619.png
IHC staining of purified anti-GFAP antibody (clone MCA-5C10) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_MCA-5C10_Purified_GFAP_Antibody_2_050619.png
    IHC staining of purified anti-GFAP antibody (clone MCA-5C10) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_MCA-5C10_Purified_GFAP_Antibody_3_050619.png
    IHC staining of purified anti-GFAP antibody (clone MCA-5C10) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_MCA-5C10_Purified_GFAP_Antibody_4_050619.png
    IHC staining of purified anti-GFAP antibody (clone MCA-5C10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_MCA-5C10_Purified_GFAP_Antibody_5_010820.png
    IHC staining of purified anti-GFAP antibody (clone MCA-5C10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No.928502), the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5_MCA-5C10_Purified_GFAP_Antibody_1_050619.png
    Western blot of purified anti-GFAP antibody (clone MCA-5C10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 40 µg of mouse brain lysate; Lane 5: 20 µg rat brain membrane lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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801103 25 µg £73
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Description

Glial Fibrillary Acidic Protein (GFAP) is a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. Astrocytes respond to many damage and disease states resulting in "astrogliosis" or the presence of a "glial response". GFAP antibodies are widely used to see the reactive astrocytes which form part of this response, since reactive astrocytes stain much more strongly with GFAP antibodies than normal astrocytes. GFAP also forms a major component of the so-called glial scar, an astrocyte rich structure apparently forming part of the barrier to nerve fiber regeneration following damage in the central nervous system. Neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of normal and reactive astrocytic cells and neural stem cells. This antibody was raised against a preparation of purified pig spinal core GFAP.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
PBS, 50% glycerol, 5mM NaN3 as supplied by vendor.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C for up to one year.
Application

IHC-P - Quality tested
WB - Verified
ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/mL for chromogenic staining and 2.0 - 10 µg/mL for fluorescent staining is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.2 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Filipowicz AR, et al. 2016. Sci Rep. 6: 32900. PubMed (IHC-P)
  2. Edamakanti CR, et al. 2018. J. Clin Invest. 128(6):2252 (ICC)
  3. de Kloet AD, et al. 2016. Brain Struct Funct. 221(2):891 (IHC-F)
  4. Hawkins KE, et al. 2014. J Neurochem. 129(1):130 (WB)
  5. Zhao WN, et al. 2012. J Biomol Screen. 17(9):1252 (ICC)
Product Citations
  1. Tao W, et al. 2020. EMBO Mol Med. 12:e12291. PubMed
RRID
AB_2810699 (BioLegend Cat. No. 801103)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of ~50 kD
Distribution

Tissue distribution:  GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.

Cellular distribution: Cytoskeleton and cytosol

Function
GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS
Antigen References
  1. van Bodegraven EJ, et al. 2019. Glia. 10.1002/glia.23594.
  2. Pekny M, et al. 2019. Neurosci Lett. 689:45.
  3. Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12).
Gene ID
2670 View all products for this Gene ID 14580 View all products for this Gene ID 24387 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Other Formats

View All GFAP Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-GFAP MCA-5C10 IHC-P,WB,ICC,IHC-F
Go To Top Version: 3    Revision Date: 10/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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