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Purified anti-GFP Antibody

Pricing & Availability
Clone
FM264G (See other available formats)
Regulatory Status
RUO
Other Names
Green Fluorescent Protein
Isotype
Rat IgG2a, κ
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Product Citations
publications
FM264G_Purified_GFP_Antibody_120618
GFP-transfected CHO cells were fixed, permeabilized, and intracellularly stained with anti-GFP (clone FM264G) Purified (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • FM264G_Purified_GFP_Antibody_120618
    GFP-transfected CHO cells were fixed, permeabilized, and intracellularly stained with anti-GFP (clone FM264G) Purified (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
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338001 25 µg £49
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338002 100 µg £89
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TLR9-GFP transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephen LA, et al. 2018. Dev. Cell. 47(1):122-132.e4. PubMed (ICC)
Product Citations
  1. Sancer G, et al. 2020. Current Biology. 29(17):2812-2825. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Sizova O, et al. 2018. Mol Cancer Res. 16:1652. PubMed
  4. Lun XK et al. 2019. Mol Cell. 74(5):1086-1102 . PubMed
  5. Luo Y, et al. 2012. J Control Release. 162:28. PubMed
  6. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  7. Zhao H, et al. 2022. EMBO Rep. 23:e52775. PubMed
  8. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  9. Wiley LA, et al. 2018. Hum Gene Ther. 29:424. PubMed
  10. Chen G, et al. 2011. J Virol. 85:11131. PubMed
  11. Zhu C, et al. 2021. Cell Reports. 34(7):108749. PubMed
  12. Sie C, et al. 2019. J Immunol. 203:1417. PubMed
  13. Bonilla WV, et al. 2021. Cell Reports Medicine. 2(3):100209. PubMed
  14. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  15. Chen AF, et al. 2022. Nat Methods. 19:547. PubMed
  16. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  17. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  18. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  19. Woo V, et al. 2019. Front Immunol. 1.061111111. PubMed
  20. Plona KL, et al. 2021. JIMD Rep. 60:56. PubMed
  21. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
RRID
AB_1279414 (BioLegend Cat. No. 338001)
AB_1279414 (BioLegend Cat. No. 338002)

Antigen Details

Biology Area
Cell Biology, Immunology
Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.

Other Formats

View All GFP Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 488 anti-GFP FM264G ICFC,ICC
Purified anti-GFP FM264G ICFC,ICC
PE anti-GFP FM264G ICFC
Alexa Fluor® 647 anti-GFP FM264G ICFC,ICC
APC anti-GFP FM264G ICFC
PerCP/Cyanine5.5 anti-GFP FM264G ICFC
PE/Cyanine7 anti-GFP FM264G ICFC
Go To Top Version: 4    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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