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Purified anti-Giantin Antibody

Pricing & Availability
Clone
Poly23A01 (See other available formats)
Regulatory Status
RUO
Other Names
Golgin subfamily B member 1, macrogolgin, golgi integral membrane protein 1, 372 kD Golgi complex-associated protein, golgin B1, golgi integral membrane protein, golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
A. Poly23A01_PURE_Giantin_ICC_090123
HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1:1000 dilution of purified anti-Giantin (clone Poly23A01), followed by incubation with Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414, orange) (panels A and B). Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) for 1 hour at room temperature and nuclei were counterstained with DAPI (Cat. No. 422801, blue) (panel B). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 25 µm
  • A. Poly23A01_PURE_Giantin_ICC_090123
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1:1000 dilution of purified anti-Giantin (clone Poly23A01), followed by incubation with Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414, orange) (panels A and B). Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) for 1 hour at room temperature and nuclei were counterstained with DAPI (Cat. No. 422801, blue) (panel B). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 25 µm
  • B. Poly23A01_PURE_Giantin_ICC_2_090123
    HeLa cells were fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1:1000 dilution of purified anti-Giantin (clone Poly23A01), followed by incubation with Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414, orange) (panels A and B) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panel B). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 25 µm
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621351 50 µL £89
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621352 200 µL £291
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Description

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant fragment of human giantin N-terminus
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution of 1:500 – 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For Immunocytochemistry (ICC), the recommended fixation/permeabilization method is 4% paraformaldehyde/0.5% Triton X-100, or paraformaldehyde followed by ice-cold methanol, or ice-cold methanol fixed only.

RRID
AB_3083408 (BioLegend Cat. No. 621351)
AB_3083408 (BioLegend Cat. No. 621352)

Antigen Details

Structure
Homodimer
Distribution

Widely express in tissues/localized to Golgi apparatus membrane

Function
Participate in forming intercisternal cross-bridges of the Golgi complex
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers
Antigen References
  1. Day PM, et al. 2013. J. Virol. 87:3862-70.
  2. Linstedt AD and Hauri HP. 1993. Mol. Biol. Cell. 4:679-93.
Gene ID
2804 View all products for this Gene ID
UniProt
View information about Giantin on UniProt.org
Go To Top Version: 1    Revision Date: 09/01/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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