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Purified anti-GSK3β Antibody

Pricing & Availability
Clone
9B1-5C3-5G8 (See other available formats)
Regulatory Status
RUO
Other Names
glycogen synthase kinase 3 beta, Serine/Threonine-Protein Kinase GSK3B, Glycogen Synthase Kinase-3 Beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9B1-5C3-5G8_PURE_GSK3B_Antibody_032122
ICC staining of purified anti-GSK3B (clone 9B1-5C3-5G8) on A431 cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were then counterstained with DAPI (panel A) (Cat. No. 422801), or not counterstained (panel B), and the image was captured with a 60X objective.
  • 9B1-5C3-5G8_PURE_GSK3B_Antibody_032122
    ICC staining of purified anti-GSK3B (clone 9B1-5C3-5G8) on A431 cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were then counterstained with DAPI (panel A) (Cat. No. 422801), or not counterstained (panel B), and the image was captured with a 60X objective.
  • 9B1-5C3-5G8_PURE_GSK3B_Antibody_2_032122
    IHC staining of purified anti-GSK3B (clone 9B1-5C3-5G8) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 μg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
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604651 25 µg £81
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604652 100 µg £230
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Description

GSK3B is a serine-threonine kinase and a member of the glycogen synthase kinase subfamily. It is a negative regulator of the hormonal control of glucose, Wnt signaling and also transcriptional factors and microtubules. It has been associated with regulation of several cellular mechanisms such as glycogen synthesis, protein synthesis, cell proliferation, microtubules stabilization, cell migration, autophagy and apoptosis. GSK3B is activated by phosphorylation at tyrosine 216 and inactivated by phosphorylation at serine 9. Defects on GSK3B has been associated with Alzheimer’s and Parkinson’s diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 4.0 - 6.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol, or fixation/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X.

For IHC-P, we recommend antigen retrieval using citrate buffer, HIER, and overnight antibody incubation at 4°C.

RRID
AB_2922641 (BioLegend Cat. No. 604651)
AB_2922641 (BioLegend Cat. No. 604652)

Antigen Details

Structure
GSK3B is a 420 amino acid protein with a mass of 46.7 kD
Distribution

GSK3B is expressed in the cytoplasm of testis, prostate, ovary and at lower levels in lung, brain and kidney

Function
GSK3B is a kinase involved in the regulation of several signaling pathways involving transcriptional factors, hormonal control of glucose, microtubule rearrangement, cell migration and proliferation, apoptosis and autophagy.
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Wu H, et al. 2019. Autophagy. 15:1506-1522.
  2. Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998.
  3. Ryu HY, et al. 2021. Exp Mol Med. 53:369-383.
  4. Myöhänen TT, et al. 2021. Basic Clin Pharmacol Toxicol. 129:287-296.
  5. Taylan E, et al. 2020. Gynecol Oncol. 159:277-284. 
  6. Mancinelli R, et al. 2017. Oxid Med Cell Longev. 2017:4629495.
Gene ID
2932 View all products for this Gene ID
UniProt
View information about GSK3beta on UniProt.org
Go To Top Version: 1    Revision Date: 03/18/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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