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Purified anti-GSK3β Phospho (Ser9) Antibody

Pricing & Availability
Clone
A21018C (See other available formats)
Regulatory Status
RUO
Other Names
Glycogen synthase kinase-3 beta; GSK3B, GSK-3B, GSK-3beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. A21018C_-Purified_-GSK3b_WB_100523
Whole cell extracts (15 µg total protein) from Jurkat cells untreated or treated with wortmannin were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-GSK3b Phospho (Ser9) (clone A21018C) (left), or anti- GSK3b Pan (right) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • a. A21018C_-Purified_-GSK3b_WB_100523
    Whole cell extracts (15 µg total protein) from Jurkat cells untreated or treated with wortmannin were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-GSK3b Phospho (Ser9) (clone A21018C) (left), or anti- GSK3b Pan (right) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • b. A21018C_-PURE_-GSK3b_WB_10052
    Whole cell extracts (15 µg total protein) from NIH/3T3 cells untreated or treated with PDGF were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-GSK3b Phospho (Ser9) (clone A21018C) (left), or anti-GSK3b Pan (right) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • c. A21018C_-Purified_-GSK3b_ICFC_060823
    Jurkat cells untreated (positive control, filled histogram) or treated with wortmannin (negative control, open histogram) (left), or NIH/3T3 cells untreated (negative control, open histogram) or treated with PDGF-BB (positive control, filled histogram) (right) were fixed and permeabilized using True-Phos™ Fix/Perm Buffer Set (Cat. No. 425401). Then, cells were intracellularly stained with purified anti-GSK3β Phospho (Ser9) (clone A21018C), or purified mouse IgG1, κ isotype control (open histogram, dashed line) (Cat. No. 400101), followed by PE goat anti-mouse IgG (Cat. No. 405307).
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618551 25 µg £113
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618552 100 µg £288
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Description

Glycogen synthase kinase 3 beta or GSK3β, is a 420 aa length constitutively active serine-threonine kinase that phosphorylates more than 100 target proteins. GSK3β is involved in several biological activities including glycogen metabolism, cell signaling, cellular transport, cell proliferation, apoptosis, and others. The activity of GSK3β is regulated by phosphorylation at different residues. Phosphorylation at tyrosine 216 enhances GSK3β kinase activity, while phosphorylation at serine 9 decreases kinase activity. In addition, GSK3β activity can also be regulated by cellular localization and/or formation of protein complexes. GSK3β has been associated with several diseases including type 2 diabetes, Alzheimer’s disease, inflammation, cancer, addiction, and bipolar disorders.  

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human GSK3β phosphorylated at Serine 9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ICFC: Cells were fixed and permeabilized using the True-Phos™ Buffer system

RRID
AB_3068059 (BioLegend Cat. No. 618551)
AB_3068059 (BioLegend Cat. No. 618552)

Antigen Details

Structure
GSK3β is a 420 a.a protein with a predicted molecular weight approximately 47 kD. GSK3β has a bi-lobar architecture, with N-terminal and C- terminal domains. The N-terminal is responsible for ATP binding, while the C-terminal carries the activation loop that mediates the kinase activity.
Distribution

GSK3β can be found mainly in the cytoplasm, however, it could also be present in mitochondria and nucleus.  

Function
It is a serine/threonine Kinase, its activity can be enhanced by phosphorylation on its tyrosine 216, or reduced by phosphorylation on its serine 9.
Interaction
Glycogen synthase, Beta-catenin/Wnt, Insulin receptor, IRS1, MAPT/Tau proteins, NF-κB, MUC1, MACF1, E2F1, mTORC2, JUN, NFATC
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family
Phospho-Proteins
Antigen References
  1. Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998.e5.
  2. Xing B, et al. 2018. Glia. 66:1999-2012.
  3. Ryu HY, et al. 2021. Exp Mol Med. 53:369-383.
  4. Wu H, et al. 2019. Autophagy. 15:1506-1522.
Gene ID
2932 View all products for this Gene ID
UniProt
View information about GSK3b Phospho (Ser9) on UniProt.org
Go To Top Version: 1    Revision Date: 06/09/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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