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Purified anti-HCN1 Antibody

Pricing & Availability
Clone
N70/28 (See other available formats)
Regulatory Status
RUO
Other Names
BCNG1; DEE24; HAC-2; BCNG-1; EIEE24; GEFSP10
Isotype
Mouse IgG1, κ
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Product Citations
publications
N70slash28_PURE_HCN1_Antibody_1_102021.png
IHC staining of anti-HCN1 antibody (clone N70/28) on formalin-fixed paraffin-embedded mouse eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
  • N70slash28_PURE_HCN1_Antibody_1_102021.png
    IHC staining of anti-HCN1 antibody (clone N70/28) on formalin-fixed paraffin-embedded mouse eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
  • N70slash28_PURE_HCN1_Antibody_2_102021.png
    IHC staining of anti-HCN1 antibody (clone N70/28) on formalin-fixed paraffin-embedded rat eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
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601051 25 µg £57
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601052 100 µg £113
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Description

Hyperpolarization-activated cyclic nucleotide-gated (HCN) 1 channel is a member in the superfamily of six-transmembrane segment channels, and related to cyclic nucleotide-gated channels and voltage-dependent K+ channels. Four isoforms, HCN1-4, have been identified with up to 60% of sequence homology. The transmembrane α-helices and C-terminal cyclic-nucleotide binding domain of HCN channels are important for K+ ions selectivity filter and cAMP modulation. HCN1 is widely expressed in the nervous system where it plays a role in regulating membrane potential, dendritic integration, neuronal pacemaking, and action potential threshold. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant rat HCN1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity of clone N70/28 with human HCN1 is observed but weaker than reactivity with mouse or rat HCN1.

RRID
AB_2904439 (BioLegend Cat. No. 601051)
AB_2904439 (BioLegend Cat. No. 601052)

Antigen Details

Structure
HCN1 is an 890 amino acid protein with a molecular mass of 99 kD.
Distribution

Membrane/ Photoreceptor cells, bipolar cells, muller glia cells, cardiomyocytes

Function
HCN1 functions as a sensor in response to voltage stimuli and cAMP modulation.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience
Molecular Family
Ion Channels
Antigen References
  1. Tanguay J, et al. 2019. Sci Rep. 9:465
  2. Ramirez D, et al. 2018. Molecules. 23:2094
  3. Lee CH and MacKinno R. 2017. Cell. 168:111-120
  4. Roubille F and Tardif JC. 2013. Circulation. 127:1986-1996 
  5. Shah MM. 2012. Sci Signal. 5:pe44
Gene ID
348980 View all products for this Gene ID
UniProt
View information about HCN1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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