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Purified anti-HDAC1 Phospho (Ser406) Antibody

Pricing & Availability
Clone
BT-15 (See other available formats)
Regulatory Status
RUO
Other Names
Histone Deactylase 1, RPD3L1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BT-15_Pure_HDAC1-phospho_Antibody_1_012319
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole for 16 hours were resolved on a 4-20% Tris-glycine gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC1 Phospho (Ser406) Antibody, clone BT-15, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 dilution. Lane M: Molecular Weight marker.
  • BT-15_Pure_HDAC1-phospho_Antibody_1_012319
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole for 16 hours were resolved on a 4-20% Tris-glycine gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC1 Phospho (Ser406) Antibody, clone BT-15, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 dilution. Lane M: Molecular Weight marker.
  • BT-15_Pure_HDAC1-phospho_Antibody_2_012319
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with Purified anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) followed by PE Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) and DAPI.
  • BT-15_Pure_HDAC1-phospho_Antibody_3_012419_updated.png
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with Purified anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE and anti-Histone H3-Phosphorylated (Ser28) Antibody (clone HTA28) Alexa Fluor® 647.
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611001 25 µg £77
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611002 100 µg £182
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Description

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Zebrafish
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.

Clone BT-15 cross-reacts to Zebrafish2

Application References

(PubMed link indicates BioLegend citation)
  1. Segre CV, et al, 2016. mAbs. 8: 37-42
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
RRID
AB_2801111 (BioLegend Cat. No. 611001)
AB_2801111 (BioLegend Cat. No. 611002)

Antigen Details

Structure
HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Segre CV, et al, 2016. mAbs. 8: 37-42.
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 Phospho Ser406 on UniProt.org
Go To Top Version: 1    Revision Date: 01/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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