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Purified anti-HDAC3 Antibody

Pricing & Availability
Clone
W15097A (See other available formats)
Regulatory Status
RUO
Other Names
Histone deacetylase 3, HD3, SMAP45, RPD3-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_W15097A_Pure_HDAC3_Antibody_2_WB_071217
Total lysates (15µg protein) from 293T and 293T/HDAC3 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-HDAC3 antibody, clone W15097A (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC3 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 1_W15097A_Pure_HDAC3_Antibody_2_WB_071217
    Total lysates (15µg protein) from 293T and 293T/HDAC3 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-HDAC3 antibody, clone W15097A (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC3 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 2_W15097A_Pure_HDAC3_Antibody_1_WB_Updated_033016
    Total cell lysate from human HeLa cells (lane 1), human MCF7 cells (lane 2), and mouse NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-HDAC3 (clone W15097A) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • 3_W15097A_Pure_HDAC3_Antibody_2_ICC_041619
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.5 µg/ml purified anti-HDAC3 (clone W15097A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated Phalloidin (red) and Alexa Fluor® 488 anti-mouse IgG antibodies (green) for one hour at room temperature. The image was captured with a 40X objective.
  • W15097A_Pure_HDAC3_Antibody_ICC_2_093020
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Mouse IgG2b, κ Isotype Control Antibody (Cat. No. 402201, panel A) or Purified anti-HDAC3 Antibody, clone W15097A (panel B), overnight at 4° C, followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG at 2.0 µg/mL (Cat. No. 405326). Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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685201 25 µg £85
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685202 100 µg £197
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Description

HDAC3, also known as RPD3-2, HDAC-3, and SMAP45, is a histone deacetylase and is responsible for the deacetylation of lysine residues on core histones and other non-histone substrates. The deacetylation of histones is important for transcriptional regulation, cell cycle progression, and other developmental related functions. HDAC3 represses gene expression by participating in the transcriptional repressor activity of BCL6, by deacetylating H3K27 on enhancer elements. It is also related to participating in transcriptional regulation through binding to YY1, which is a a zinc-finger transcription factor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HDAC3 recombinant protein (405-424 a.a.) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2572203 (BioLegend Cat. No. 685201)
AB_2572203 (BioLegend Cat. No. 685202)

Antigen Details

Structure
428 amino acids with a predicted molecular weight of 49 kD. It belongs to the class I histone deacetylase family.
Distribution

Nucleus.

Function
HDAC3 is a deacetylase responsible for the deacetylation of lysine resides on core histones and other non-histone proteins.
Interaction
Forms heterodimer with YY1. Interacts with HDAC7, 9, 10, DACH1, DAXX, BCOR, MJD2A/JHDM3A, NRIP1, PRDM6, and SRY.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Hua G, et al. 2016. Proc Natl Acad Sci USA. 113:E626.
2. Choi HK, et al. 2015. Nat. Commun. 6:8225.
3. Kim WY, et al. 2015. Int. J. Mol. Med. 35(1):202-10.
4. McHugh CA, et al. 2015. Nature 521:232.
5. Sun Z, et al. 2013. Mol. Cell. 52:769.
6. Sun Z, et al. 2012. Nat. Med. 18:934.

Gene ID
8841 View all products for this Gene ID
UniProt
View information about HDAC3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/30/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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