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Purified anti-HDAC4 Antibody

Pricing & Availability
Clone
14A9C47 (See other available formats)
Regulatory Status
RUO
Other Names
Histone deacetylase 4, HD4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
14A9C47_Purified_HDAC4_Antibody_WB_081417
Total lysates (15 µg protein) from human platelet and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-HDAC4 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone POLY4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
  • 14A9C47_Purified_HDAC4_Antibody_WB_081417
    Total lysates (15 µg protein) from human platelet and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-HDAC4 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone POLY4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
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676801 25 µg £85
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676802 100 µg £197
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Description

HDAC4 is a member of the class II histone deacetylase family and is similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 has been shown to play a crucial role in skeletal formation. HDAC4 prevents chondrocyte hypertrophy through inhibiting Runx2 activity during endochondral bone formation. The HDAC4 deficient mice show chondrocyte hypertrophy and premature ossification of developing bones. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human HDAC4 recombinant protein (529 - 651 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 14A9C47 weakly cross-reacts with mouse HDAC4.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

In some experimental systems a non-specific band may be observed at ~75kD.




 

RRID
AB_2861052 (BioLegend Cat. No. 676801)
AB_2861052 (BioLegend Cat. No. 676802)

Antigen Details

Structure
1084 amino acids with a predicted molecular weight of 119 kD. Contains a histone deacetylase region and a N-terminal homodimerization domain.
Distribution

Cytoplasm and nucleus.

Interaction
CDKN2A forms a homodimer and interacts with HDAC7, MEF2C, BTBD14B, AHRR, NR2C1, adn 14-3-3.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Antigen References

1. Tong JK, et al. 1998. Nature 395:917.
2. Grunstein M.  1997. Nature 389:349.
3. Wolffe AP. 1996. Science 272:371.
4. Lee DY, et al. 1993. Cell 72:73.
5. Wang AH, et al. 2001. Mol. Cell Biol. 21:5992.
6. Bertos NR, et al. 1999. Mol. Cell Biol. 11:7816.

Gene ID
9759 View all products for this Gene ID
UniProt
View information about HDAC4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/09/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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