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Purified anti-HDAC7 Antibody

Pricing & Availability
Clone
11C7C49 (See other available formats)
Regulatory Status
RUO
Other Names
Histone deacetylase 7, HD7, HDAC7a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_11C7C49_PURE_HDAC7_Antibody_Antibody_1_102218
Total cell lysates (15 µg total protein) from HeLa, Jurkat, and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC7 Antibody, clone 11C7C49, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_11C7C49_PURE_HDAC7_Antibody_Antibody_1_102218
    Total cell lysates (15 µg total protein) from HeLa, Jurkat, and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC7 Antibody, clone 11C7C49, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_11C7C49_GoChIP_HDAC7_Antibody_042617.jpg
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC7 Antibody (Clone 11C7C49), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-HDAC7 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human NucE gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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683502 100 µg £213
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Description

Histone deacetylases (HDACs) regulate a variety of biological processes by deacetylating lysine residues in the N-terminal tails of core histones and non-histone proteins. HDAC7 is a class IIa histone deacetylase and its enzymatic activity is dependent on the interaction with HDAC3. HDAC7 is highly expressed in CD4+CD8+ double-positive thymocytes and has been suggested to repress the expression of Nur77 through the transcription factor MEF2D. HDAC7 is mostly localized to the nucleus and can be sequestrated in the cytoplasm by interacting with 14-3-3 protein during T cell receptor activation. The nuclear export of HDAC7 leads to the expression of Nur77 and apoptosis. HDAC7 has been shown to regulate the maturation of osteoclast and osteoblast, as well as the development of muscle and blood vessel.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic HDAC7 peptide conjugated to KLH (1-17 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572024 (BioLegend Cat. No. 683502)

Antigen Details

Structure
952 amino acids with a predicted molecular weight of 102.9 kD. Contains a histone deacetylase domain.
Distribution

Cytoplasm and the nucleus.

Function
HDAC7 mediates deacetylation of lysine residues in the N-terminal tails of core histones. It plays important roles in the regulation of T-cell differentiation and development of muscle and blood vessel.
Interaction
HDAC7 interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, FOXP3, KAT5, EDNRA, KDM5B, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Barneda-Zahonero B, et al. 2013. PLoS Genet. 9:e1003503.
2. Pham L, et al. 2011. J. Biol. Chem. 286:12056.
3. Li S, et al. 2011. Mol. Cancer 10:18.
4. Gao C, et al. 2010. Biochim. Biophys. Acta. 1803:1186.
5. Margariti A, et al. 2009. J. Cell. Sci. 122:460.
6. Parra M, et al. 2007. Genes Dev. 21:638.

Gene ID
51564 View all products for this Gene ID
UniProt
View information about HDAC7 on UniProt.org
Go To Top Version: 5    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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