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Purified anti-Hexokinase II Antibody

Pricing & Availability
Clone
W19163B (See other available formats)
Regulatory Status
RUO
Other Names
HKII, Muscle Form Hexokinase
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19163B_PURE_HexokinaseII_Antibody_121420
Whole cell extracts (15 µg total protein) from A549 (negative control), HeLa, U87-MG, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Hexokinase II antibody (clone W19163B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19163B_PURE_HexokinaseII_Antibody_121420
    Whole cell extracts (15 µg total protein) from A549 (negative control), HeLa, U87-MG, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Hexokinase II antibody (clone W19163B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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944001 25 µg £81
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944002 100 µg £201
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Description

Hexokinase enzymes are evolutionarily conserved proteins that play a critical role in glycolysis by catalyzing the conversion of glucose to the intermediate glucose-6-phosphate. In humans, four hexokinase isozymes exist that differ in their tissue distribution and affinity for glucose. The hexokinase II isozyme is ubiquitously expressed and is the most highly regulated member of the hexokinase family. In addition to its cytosolic localization, hexokinase II also associates with the mitochondrial outer membrane, resulting in efficient substrate channeling of ATP to the enzyme which fuels cell proliferation. Hexokinase II association with the outer mitochondrial membrane protein VDAC disrupts the ability of the pro-apoptotic protein Bax to induce mitochondrial dysfunction and cell death through cytochrome C release. Many cancer cells show elevated expression of hexokinase II, which allows efficient funneling of glucose to support anaerobic glycolysis, as well as increased survival through inhibition of Bax-mediated apoptotic death.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant hexokinase II protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

-This clone was tested for ICC on HeLa cells fixed and permeabilized with 4% PFA plus Trixon X-100, 4% PFA plus methanol, or methanol. The former two methods resulted in nuclear staining, inconsistent with the published cytosolic and mitochondrial outer membrane-associated localization of hekokinase II. The latter method (methanol only) resulted in hexokinase II staining, but the signal observed did not meet our sensitivity criteria. We therefore do not recommend this clone for the application.

-This clone failed to immunoprecipitate hexokinase II from HeLa extracts. We therefore do not recommend this clone for the application.

RRID
AB_2890868 (BioLegend Cat. No. 944001)
AB_2890868 (BioLegend Cat. No. 944002)

Antigen Details

Structure
Hexokinase II is a 917 amino acid protein with a predicted molecular weight of 102 kD.
Distribution

Ubiquitously expressed; highly expressed in muscle and adipose tissue/Cytosolic, outer mitochondrial membrane-associated

Function
Glycolysis
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References

1. Pastorino JG, et al. 2002. J. Biol. Chem. 277:7610
2. Majewski N, et al. 2004. Mol. Cell. 16:819

Gene ID
3099 View all products for this Gene ID
UniProt
View information about Hexokinase II on UniProt.org
Go To Top Version: 1    Revision Date: 12/14/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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