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Purified anti-HGF Antibody

Pricing & Availability
Clone
A15061E (See other available formats)
Regulatory Status
RUO
Other Names
Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15061E_PURE_HGF_Antibody_101716
Total cell lysates (30 µg protein) from U87-MG (lane 1), THP-1 untreated (lane 2), THP-1 treated with PMA for three days and LPS for one day (lane 3), Raw264.7 (lane 4) and 5 ng recombinant human HGF (lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis under non-reducing condition, transferred to nitrocellulose, and probed with purified anti-HGF (clone A15061E) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • A15061E_PURE_HGF_Antibody_101716
    Total cell lysates (30 µg protein) from U87-MG (lane 1), THP-1 untreated (lane 2), THP-1 treated with PMA for three days and LPS for one day (lane 3), Raw264.7 (lane 4) and 5 ng recombinant human HGF (lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis under non-reducing condition, transferred to nitrocellulose, and probed with purified anti-HGF (clone A15061E) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • A15061E_PURE_HGF_Antibody_2_031720
    Whole cell extracts (15 µg total protein) of indicated cell lines, reduced or non-reduced with βME, were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 mg/mL of purified anti-HGF antibody (clone A15061E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
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692602 100 µg £174
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Description

HGF (Hepatocyte growth factor) is a multifunctional cytokine that plays important roles in cell proliferation, survival, motility and morphogenesis. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases; this protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules. Secreted by cells of mesenchymal origin, HGF is the specific ligand for the tyrosine-kinase receptor c-MET (cellular mesenchymal-epithelial transition), also called MET, which is expressed in different types of epithelial, endothelial and hematopoietic progenitor cells. Aberrantly activated MET/HGF signaling correlates with tumorigenesis and metastasis, and is regarded as a target for novel anti-cancer treatments.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human HGF (carrier-free)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody works for Western blotting under non-reducing conditions only and does not cross-react with mouse.

RRID
AB_2632766 (BioLegend Cat. No. 692602)

Antigen Details

Structure
Heterodimer (alpha and beta chains). Pro-HGF has 728 amino acids, the predicted molecular weight is approximately 83 kD. The alpha and beta chains contain 463 and 234 amino acids and have a predicted molecular mass of approximately 53.6 and 26 kD respec
Distribution

Mesenchymal cells, liver sinusoidal endothelial, and macrophages.

Function
Stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. Also, TGF-β and angiotensin-II depress HGF production in vitro.
Interaction
Hepathocytes and epithelial cells.
Ligand/Receptor
MET receptor.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Hartmann S, et al. 2016. Clin. Cancer Res. 22:4005.
2. Fajardo-Puerta AB, et al. 2016. J. Clin. Pathol. 69:575.
3. Zhang Y, et al. 2016. Oncotarget. 7:37370-89.
4. Frisch RN, et al. 2016. Expert Opin. Ther. Targets 20:1087.
5. Kataoka H, et al. 2000. J. Biol. Chem. 275:40453.
6. Wang X, et al. 2002. Mol. Cell. 9:411.
7. Wu MH, et al. 2004. Gene Ther. 11:170.
8. Mizuno S, et al. 2008. Front. Biosci. 13:7072.
9. D'Angelo F, et al. 2013. Clin. Exp. Immunol. 174:60.

Gene ID
3082 View all products for this Gene ID
UniProt
View information about HGF on UniProt.org
Go To Top Version: 2    Revision Date: 03/17/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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