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Purified anti-HIF2A Antibody

Pricing & Availability
Clone
W22144B (See other available formats)
Regulatory Status
RUO
Other Names
Q99814, Hypoxia induficble factor 2-alpha (HIF2A, HIF2α), Endothelial PAS domain-containing protein 1 (EPAS1), class E basic helix-loop-helix protein 73 (bHLHe73), Member of PAS protein 2 (MOP2), PAS domain-containing protein 2 (PASD2), BHLHE73, HIF2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22144B_PURE_-HIF2A_WB_111124
Whole cell extracts (15 µg total protein per lane) from HeLa cells untreated (-) or treated (+) with CoCl2 (200 µM, 18 hours) to induce hypoxia-like conditions and stabilize HIF2A were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HIF2A (clone W22144B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22144B_PURE_-HIF2A_WB_111124
    Whole cell extracts (15 µg total protein per lane) from HeLa cells untreated (-) or treated (+) with CoCl2 (200 µM, 18 hours) to induce hypoxia-like conditions and stabilize HIF2A were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HIF2A (clone W22144B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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677051 25 µg £100
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677052 100 µg £284
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Description

HIF2A is a transcription factor that mediates the cellular adaptive response to hypoxic conditions, orchestrating the transcription of a wide array of target genes to enable cellular survival and homeostatic maintenance in low-oxygen environments. At an organismal level, HIF2A is also involved in essential physiological processes such as erythropoiesis and angiogenesis. Under normoxic conditions, HIF2A undergoes prolyl hydroxylation which leads to its degradation by the proteasome. Oxygen starvation inhibits prolyl hydroxylation and prevents HIF2A degradation, leading to stabilization of the protein and translocation to the nucleus to alter gene expression. Dysregulation of HIF2A has been implicated in various pathological states including cancer, where it promotes tumor growth by enhancing the ability of tumor cells to survive hypoxic conditions within the tumor microenvironment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the C-terminus of human HIF2A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or immunohistochemistry in formalin-fixed, paraffin-embedded tissues (IHC-P).

Antigen Details

Structure
HIF2A is an 870 amino acid protein with a predicted molecular weight of 96.5 kD.
Distribution

Cytoplasmic, translocates to nucleus upon stabilization

Function
Transcription factor
Interaction
Dimerizes with HIF1B upon hypoxic induction. Under normoxic conditions, undergoes prolyl hydroxylation by Von Hippel-Lindau (VHL)
Cell Type
Endothelial cells
Biology Area
Signal Transduction, Transcription Factors
Antigen References
  1. Tian H, et al. 1997. Genes Dev. 11:72-82. 
  2. Wiesener, et al. 1998. Blood. 92:2260-8. 
  3. Giatromanolaki A and Harris AL 2001. Anticancer Res. 6B:4317-24. 
  4. MacInnis MJ and Rupert JL. 2011. High Alt Med Biol. 12:133-9.
Gene ID
2034 View all products for this Gene ID
UniProt
View information about HIF2A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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