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Purified anti-Histone H4 Trimethyl (Lys 20) Antibody (Previously Covance catalog# MMS-5288)

Pricing & Availability
Clone
6F8-D9 (See other available formats)
Regulatory Status
RUO
Other Names
Histone H4, histone 1, H4a, H4 histone family, member A, H4K20me3
Previously
Covance Catalog# MMS-5288
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_6F8-D9_Purified_Histone-H4_Antibody_IHC_022215
IHC staining of purified anti-Histone H4 Trimethyl (Lys 20) antibody (clone 6F8-D9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_6F8-D9_Purified_Histone-H4_Antibody_IHC_022215
    IHC staining of purified anti-Histone H4 Trimethyl (Lys 20) antibody (clone 6F8-D9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_6F8-D9_PURE_Histone_H4_Trimethyl_Antibody_WB_120517
    Western blot of purified anti-Histone H4 Trimethyl (Lys 20) antibody (clone 6F8-D9). Lane M: Molecular weight marker; Lane 1: 15 µg of Jurkat cell lysate; Lane 2: 15 µg of RAW264.7 cell lysate; Lane 3: 15 µg of UMR106 cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (bottom blot). The bands were visualized using chemiluminescence detection.
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827701 100 µL £189
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Botuyan MV, et al. 2006. Cell. 127:1361. (WB)
Product Citations
  1. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
RRID
AB_2564914 (BioLegend Cat. No. 827701)

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Histone H4 is ubiquitously expressed in all cells.

Function
Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
Interaction
Various transcription factors.
Biology Area
Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
2. Bhasin M, et al. 2006. J. Comput. Biol. 13:102.
3. Nelson DM, et al. 2016. Genome Biol. 17:158. 

Gene ID
8359 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 8    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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