Western blot of purified anti-HNRNPA1 antibody (4B10/HNRNPA1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blots were incubated with 0.5 µg/mL of 4B10/HNRNPA1 or mouse IgG2a overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
Western blot of purified anti-HNRNPA1 antibody (4B10/HNRNPA1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blots were incubated with 0.5 µg/mL of 4B10/HNRNPA1 or mouse IgG2a overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 250 µm
IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IIHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multi-purpose RNA binding protein involved in RNA metabolism and DNA genomic stability in normal and pathological conditions. hnRNP A1 is ubiquitously expressed and usually associates with nascent RNA polymerase II transcripts. hnRNP A1 is abundantly expressed in neuronal cells and reproductive tissue. Loss of hnRNP A1 has been observed in several neurodegenerative diseases, including Alzheimer’s disease, multiple sclerosis, amytrophic lateral sclerosis. Structurally, hnRNPA1 has two RNA recognition motifs, which are post-translationally modified to alter its nuclear pore shuttling properties and RNA interaction.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.1 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
hnRNPA1 has two isoforms. Human HNRPNA1 isoform A1-A is a 320 amino acid protein with a molecular mass of 34 kD, and isoform A1-B is a 372 amino acid protein with a molecular mass of 38 kD.
Distribution
Tissue Distribution: Ubiquitous expression
Cellular Source: Nucleus and cytoplasm
Function
RNA metabolism, genomic stability
Interaction
Nascent RNA polymerase II transcripts
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References
Bekenstein U, et al. 2013. Mol Cell Neurosci. 56:436-46.
Jean-Philippe J, et al. 2014. Biochim Biophys Acta. 1839(4): 251–258.
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