Purified anti-HNRNPA1 Antibody

Pricing & Availability
Clone
4B10/HNRNPA1 (See other available formats)
Regulatory Status
RUO
Other Names
Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
4B10-HNRNPA1_Pure_HNRNPA1_Antibody_1_040119
Western blot of purified anti-HNRNPA1 antibody (4B10/HNRNPA1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blots were incubated with 0.5 µg/mL of 4B10/HNRNPA1 or mouse IgG2a overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_1_040119
    Western blot of purified anti-HNRNPA1 antibody (4B10/HNRNPA1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blots were incubated with 0.5 µg/mL of 4B10/HNRNPA1 or mouse IgG2a overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_2_040119
    IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_3_040119
    IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 250 µm
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_4_040119
    IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_5_040119
    IIHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4B10-HNRNPA1_Pure_HNRNPA1_Antibody_6_040119
    IHC staining of purified anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
Compare all formats
Cat # Size Price Quantity Check Availability Save
862501 25 µg £73
Check Availability


Need larger quantities of this item?
Request Bulk Quote
862502 100 µg £189
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multi-purpose RNA binding protein involved in RNA metabolism and DNA genomic stability in normal and pathological conditions. hnRNP A1 is ubiquitously expressed and usually associates with nascent RNA polymerase II transcripts. hnRNP A1 is  abundantly expressed in neuronal cells and reproductive tissue. Loss of hnRNP A1 has been observed in several neurodegenerative diseases, including Alzheimer’s disease, multiple sclerosis, amytrophic lateral sclerosis. Structurally, hnRNPA1 has two RNA recognition motifs, which are post-translationally modified to alter its nuclear pore shuttling properties and RNA interaction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified HeLa HNRNPA1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.1 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801243 (BioLegend Cat. No. 862501)
AB_2801243 (BioLegend Cat. No. 862502)

Antigen Details

Structure
hnRNPA1 has two isoforms. Human HNRPNA1 isoform A1-A is a 320 amino acid protein with a molecular mass of 34 kD, and isoform A1-B is a 372 amino acid protein with a molecular mass of 38 kD.
Distribution

Tissue Distribution: Ubiquitous expression
Cellular Source: Nucleus and cytoplasm

Function
RNA metabolism, genomic stability
Interaction
Nascent RNA polymerase II transcripts
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References
  1. Bekenstein U, et al. 2013. Mol Cell Neurosci. 56:436-46.
  2. Jean-Philippe J, et al. 2014. Biochim Biophys Acta. 1839(4): 251–258.
Gene ID
3178 View all products for this Gene ID 15382 View all products for this Gene ID 29578 View all products for this Gene ID
UniProt
View information about HNRNPA1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All HNRNPA1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-HNRNPA1 4B10/HNRNPA1 WB,IHC-P
HRP anti-HNRNPA1 4B10/HNRNPA1 WB,IHC-P
Biotin anti-HNRNPA1 4B10/HNRNPA1 IHC-P
Alexa Fluor® 488 anti-HNRNPA1 4B10/HNRNPA1 IHC-P
Alexa Fluor® 594 anti-HNRNPA1 4B10/HNRNPA1 IHC-P
Alexa Fluor® 647 anti-HNRNPA1 4B10/HNRNPA1 IHC-P
Go To Top Version: 1    Revision Date: 04/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account