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Purified anti-hnRNPK Antibody

Pricing & Availability
Clone
F45P9C7 (See other available formats)
Regulatory Status
RUO
Other Names
Heterogeneous nuclear ribonucleoprotein K, DC-stretch binding protein, Transformation upregulated protein (TUNP), hnRNP K, hnRNP-K
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_F45P9C7_PURE_hnRNPK_WB_050118
Total lysates (15 µg protein) from HepG2, Daudi and UMR106 (rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • A_F45P9C7_PURE_hnRNPK_WB_050118
    Total lysates (15 µg protein) from HepG2, Daudi and UMR106 (rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • B_F45P9C7_PURE_hnRNPK_Antibody_WB_081616
    Total cell lysate from HeLa cells (lane 1, 15 µg), 293E cells (lane 2, 15 µg), U87MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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634601 25 µg £77
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634602 100 µg £157
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Description

hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. This protein containing KH domains and is ubiquitously expressed in the cytoplasm and the nucleus with a predicted molecular weight approximately 51 kD. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling, and is modified by phosphorylation (PKC δ, c-Src) and acetylation. Clone F45P9C7 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, rabbit and chicken hnRNP K.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide SVKQYSGKFF
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed
  2. Carpenter B, et al. 2007. Br J Cancer. 97:574. PubMed
  3. Alnabulsi A, et al. 2009. Methods of cancer diagnosis, therapy and diagnosis. 4:25.
Product Citations
  1. Carpenter B, et al. 2006. Br J Cancer. 95:921. PubMed
  2. Roychoudhury P, Chaudhuri K 2007. Br J Cancer. 97:574. PubMed
RRID
AB_2120361 (BioLegend Cat. No. 634601)
AB_2120361 (BioLegend Cat. No. 634602)

Antigen Details

Structure
Heterogenous nuclear ribonucleoprotein family, KH domains; 51 kD
Distribution

Ubiquitously expressed cytoplasmic and nuclear protein

Function
Pre-mRNA binding protein, component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. Acts as transcriptional regulator and functions in nucleocytoplasmic shuttling
Interaction
Interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, Hepatitis virus core protein
Modification
Phosphorylation (PKC δ, c-Src), Acetylation, Methylation
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Matunis MJ, et al. 1992. Mol. Cell. Biol. 12:164.
2. Dejgaard K, et al. 1994. J. Mol. Biol. 236:33.
3. Mandal M, et al. 2001. J. Biol. Chem. 276:9699

Regulation
Epidermal growth factor (EGF) can increase expression and function
Gene ID
3190 View all products for this Gene ID
UniProt
View information about hnRNPK on UniProt.org
Go To Top Version: 5    Revision Date: 05/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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