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- Clone
- 3E2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Heat shock factor protein 2, HSF 2, HSTF 2, heat shock transcription factor 2
- Previously
-
Covance Catalog# MRT-5114
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-HSF2 antibody (clone 3E2). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 829301 | 100 µL | £189 | ||||
Heat shock factor protein 2 (HSF2) is a transcription factor that enters the nucleus and binds to heat shock factor promoter elements, consisting of a consensus tandem array sequence of three oppositely oriented "AGAAN" motifs, during heat shock in eukaryotes. Under normal conditions, it exists as a homodimer in the cytoplasm of cells where it binds to heat shock proteins and inactivates them. During high temperature cellular stress, misfolding of proteins occurs, which causes heat shock proteins to dissociate from the heat shock factor proteins and bind to the misfolded proteins. This leads to free heat shock proteins, which can then enter the nucleus and activate transcription.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- This monoclonal antibody was raised against mouse recombinant HSF-2 protein.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB).
-
Application References
(PubMed link indicates BioLegend citation) -
- Sistonen L, et al. 1992. Mol Cell Biol. 12(9): 4104-11.
- RRID
-
AB_2564928 (BioLegend Cat. No. 829301)
Antigen Details
- Structure
- Expected MW: 70 kD
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Heat Shock Proteins
- Gene ID
- 3298 View all products for this Gene ID
- UniProt
- View information about HSF2 on UniProt.org
Other Formats
View All HSF2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-HSF2 | 3E2 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-HSF2
Western blot of purified anti-HSF2 antibody (clone 3E2). Lan...
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