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Purified anti-Hsp90α/β Antibody

Pricing & Availability
Clone
K3720A (See other available formats)
Regulatory Status
RUO
Other Names
Heat Shock Protein 90kD Alpha (Cytosolic), Heat Shock 90 kD Protein 1, Beta, Class
Isotype
Mouse IgG1
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Product Citations
publications
K3720A_HSP90ab_Purified_1_WB_080316
Total cell lysate from HeLa cells (lane 1, 15 µg), NIH3T3 cells (lane 2, 15 µg) and mouse brain (lane 3, 15 µg) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-HSP90α/β antibody (clone K3720A). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • K3720A_HSP90ab_Purified_1_WB_080316
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIH3T3 cells (lane 2, 15 µg) and mouse brain (lane 3, 15 µg) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-HSP90α/β antibody (clone K3720A). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • K3720A_HSP90ab_Purified_2_IF_082714
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Cells were stained with 2 µg/ml anti-HSP90α/β (clone K3720A) in blocking buffer overnight at 4°C, followed by DyLight™ 594 (red) conjugated anti-mouse IgG for two hours at room temperature. Cells were then stained with Alexa Fluor® 488 Phalloidin (green) for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The
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661802 100 µg £182
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Description

Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of a diverse set of critical factors, including regulatory, metabolic, organizational, and signaling proteins. There are two major isoforms: α and β. In humans, the Hsp90β isoform is constitutively expressed, whereas the Hsp90α isoform is expressed under stress conditions. The Hsp90 dimer acts in concert with other chaperones and is regulated by a number of co-chaperones/accessory proteins. Upregulation of Hsp90 is associated with tumor progression, invasion and formation of metastases, and development of drug resistance. Therefore Hsp90 has emerged as an important target for cancer treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 291-304 of human HSP90α and β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  1. Matsusaka T. 1996. Jpn. J. Oral. Biol. 38:633.
  2. Nemoto T, et al. 1997. J. Biol. Chem. 272:26179. (ELISA)
  3. Nemoto T, et al. 1997. Biochem. Mol. Biol. Int. 42:881.
Product Citations
  1. Zubiaga L, et al. 2023. iScience. 26:106057. PubMed
RRID
AB_2564179 (BioLegend Cat. No. 661802)

Antigen Details

Structure
Hsp90α: 732 amino acids, predicted molecular weight of approximately 85 kD. Hsp90ß: 724 amino acids, predicted molecular weight of approximately 83 kD.
Distribution

Cytoplasmic and nuclear.

Function
Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of a diverse set of critical factors, including regulatory, metabolic, organizational, and signaling proteins.
Interaction
NR3C1, FKBP4, FKBP5, PPID, PPP5C, STIP1, AHSA1, FNIP1, HSF1, SMYD3, TOM34, TERT, PTGES3, CHORDC1, DNAJC7, STUB1, UBE2N, PPP5C, PPP5C, p53/TP53 and CDK6
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
Antigen References

1. Barrott JJ, et al. 2013. FEBS J. 280:1381.
2. Gorska M, et al. 2012. Front. Biosci. (Landmark Ed) 17:2269.
3. Barginear MF, et al. 2008. Curr. Cancer Drug Targets. 8:522.
4. Arya R, et al. 2007. J. Biosci. 32:595.
5. Neckers L, et al. 2003. Curr. Opin. Oncol. 15:419.
6. Nemoto T, et al. 1997. J. Biol. Chem. 272: 26179.

Gene ID
3320 View all products for this Gene ID 3326 View all products for this Gene ID
UniProt
View information about Hsp90alpha/beta on UniProt.org
Go To Top Version: 4    Revision Date: 08/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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