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Purified anti-HSPH1 Antibody

Pricing & Availability
Clone
W20247H (See other available formats)
Regulatory Status
RUO
Other Names
Heat Shock Protein Family H Member 1, HSP110, HSP105, Heat Shock 105kDa/110kDa Protein 1, HSP105A, HSP105B, Antigen NY-CO-25, NY-CO-25
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W20247H_PURE_HSPH1_WB_110724
Whole cell lysates from the indicated cell lines (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-HSPH1 (clone W20247H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W20247H_PURE_HSPH1_WB_110724
    Whole cell lysates from the indicated cell lines (15 µg total protein) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-HSPH1 (clone W20247H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W20247H_PURE_HSPH1_IHC-P_110724
    IHC staining with purified anti-HSPH1 (clone W20247H) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with (panels A and C) and without (panel B and D) purified anti-HSPH1 (clone W20247H) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged with DAPI (panels C and D). Scale bar: 50 µm
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678451 25 µg £107
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678452 100 µg £305
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Description

The HSPH1 protein, also known as heat shock protein 105 (HSP105), is a member of the heat shock protein (HSP) family, which plays a crucial role in the maintenance of cellular homeostasis, especially under stress conditions. HSPH1 is a prominent member of the HSP110 family, which is closely related to the HSP70 family but has unique properties and functions. HSPH1 helps to mitigate these harmful effects by functioning as a molecular chaperone, ensuring that denatured proteins refold correctly or are directed towards degradation pathways. This protective mechanism is vital for preventing the accumulation of protein aggregates, which can be toxic and lead to cell death. Studies have shown that HSPH1 can interact with other heat shock proteins, such as HSP70 and HSP40, to form a functional complex that enhances its chaperone activity. Through these interactions, HSPH1 not only prevents protein misfolding but also facilitates the disaggregation of protein aggregates, promoting cellular recovery from stress.

HSPH1 is also implicated in various diseases, particularly those associated with protein misfolding and aggregation. For example, neurodegenerative disorders like Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease are characterized by the accumulation of misfolded protein aggregates in neurons. The upregulation of HSPH1 has been suggested as a protective mechanism in these conditions, as it may help reduce the burden of toxic aggregates. HSPH1 has been found to be overexpressed in various cancers, including breast, lung, and colon cancers, where it contributes to the stabilization of oncogenic proteins and supports the survival of cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human HSPH1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

It is not recommended to use this product in immunocytochemistry (ICC).

Antigen Details

Structure
HSPH1 is an 858 amino acid length protein with a predicted molecular weight of 96.9 kD. The expected molecular weight in SDS-PAGE gel is at 110 kD.
Distribution

Expressed in cytosol in most tissues

Function
Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release
Interaction
Interacts with HSPA8/HSC70, HSPA1A, and HSPA1B
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family
Heat Shock Proteins
Antigen References
  1. Easton D P, et al. 2000. Cell Stress Chaperones. 5(4):276-90.
  2. Schuermann J P, et al. 2008. Mol. Cell. 31(2):232-43.
  3. Canpanella C, et al. 2018. Int. J. Mol. Sci. 19(9):2603
Gene ID
10808 View all products for this Gene ID
UniProt
View information about HSPH1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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