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Purified anti-human ATG3 Antibody

Pricing & Availability
Clone
A19237B (See other available formats)
Regulatory Status
RUO
Other Names
Autophagy Related 3
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A19237B_PURE_ATG3_Antibody_052322
Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ATG3 (clone A19237B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A19237B_PURE_ATG3_Antibody_052322
    Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ATG3 (clone A19237B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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379702 100 µg £197
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Description

Human autophagy related 3, ATG3, is a 314 amino acid protein with a molecular mass of 36 kD, which is a ubiquitin-like modifier similar to the canonical E2 enzyme. ATG3 is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy is the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway which plays an important role in controlling normal growth but may be defective in tumor cells. ATG3 is essential for autophagocytosis, which is a starvation-induced process responsible for transport of cytoplasmic proteins to the lysosome/vacuole. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922609 (BioLegend Cat. No. 379702)

Antigen Details

Structure
Human ATG3 is a 314 amino acid protein with a molecular mass of 36 kD
Distribution

Expressed in testis and bone marrow stromal cell

Function
E2-conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2, or MAP1LC3A).
Interaction
Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
Ligand/Receptor
ATG8
Biology Area
Cell Biology, Cell Death, Immunology
Molecular Family
Apolipoproteins, Autophagosome Markers
Antigen References
  1. Isei T, et al. 2002. J Biol Chem. 277:13739.
  2. Silke M. et al. 2009. Hum Genet. 128:453.
  3. Ozlem O, et al. 2012. Apoptosis. 17:810.
Gene ID
64422 View all products for this Gene ID
UniProt
View information about ATG3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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