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Purified anti-human Cadherin 11 Antibody

Pricing & Availability
Clone
16G5 (See other available formats)
Regulatory Status
RUO
Other Names
OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
Isotype
Mouse IgG1, κ
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Product Citations
publications
16G5_Pure_Cadherin11_Antibody_1_FC_020816
Human lung cancer cell line H460 cells were stained with purified Cadherin 11 (clone 16G5) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 16G5_Pure_Cadherin11_Antibody_1_FC_020816
    Human lung cancer cell line H460 cells were stained with purified Cadherin 11 (clone 16G5) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 16G5_Pure_Cadherin11_Antibody_2_ICC_051019
    Immunofluorescence analysis. H460 cells were fixed with 4% paraformaldehyde (PFA) and blocked with 5% FBS. Then the cells were stained with 5 µg/ml anti-Cadherin 11 antibody followed by 2 µg/mL Alexa Fluor® 594 conjugated anti-mouse IgG. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 60X objective.
  • 16G5_Pure_Cadherin11_Antibody_3_WB_051019
    Protein (15 µg) harvested from HeLa, PC3, H460, and NIH3T3 was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal Cadherin 11 (clone 16G5) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) antibody and chemiluminescence detection.
  • 16G5_Pure_Cadherin11_Antibody_4_IHCP_051019
    Human paraffin-embedded ovary tissue slice was stained with purified anti-human Cadherin 11antibody overnight followed by Alexa Fluor® 594 goat anti-mouse secondary antibody (red). The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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368702 100 µg £182
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB, IHC-P - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml. For immunocytochemistry, a concentration rage of 2.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Product Citations
  1. Wei Z, et al. 2022. Cells. 11: . PubMed
RRID
AB_2566264 (BioLegend Cat. No. 368702)

Antigen Details

Structure
Type I single pass transmembrane glycoprotein.
Distribution

Osteoblasts, neuroblasts, and some cancer cells.

Function
Cell adhesion.
Interaction
Cadherin 2 (CDH2).
Ligand/Receptor
Calcium.
Cell Type
Osteoclasts
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35.
2. Christofori G, et al. 2003. EMBO. J. 22:2318.
3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092.

Gene ID
1009 View all products for this Gene ID
UniProt
View information about Cadherin 11 on UniProt.org
Go To Top Version: 1    Revision Date: 02/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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