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Purified anti-human CD127 (IL-7Rα) Recombinant Antibody

Pricing & Availability
Clone
QA18A44 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A44_PURE_CD127_Antibody_120320
Human peripheral blood lymphocytes were stained with purified anti-human CD127 (IL-7R) recombinant (clone QA18A44) (left) or purified mouse IgG1a, k isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with CD3 APC.
  • QA18A44_PURE_CD127_Antibody_120320
    Human peripheral blood lymphocytes were stained with purified anti-human CD127 (IL-7R) recombinant (clone QA18A44) (left) or purified mouse IgG1a, k isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with CD3 APC.
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375702 100 µg £85
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

QA18A44 clone of antibody is able to completely block the binding of clone A019D5 to IL-7Rα.

This recombinant antibody clone is derived from clone A019D5.

RRID
AB_2888838 (BioLegend Cat. No. 375702)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Interaction
CD132
Ligand/Receptor
IL-7
Cell Type
B cells, Lymphocytes, NK cells, T cells, Tregs
Biology Area
Cell Proliferation and Viability, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 2    Revision Date: 12/03/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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