- Clone
- DL-101 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V B045
- Other Names
- Syndecan-1, B-B4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![DL-101_Purified_CD138_Cytof_060314.jpg DL-101_Purified_CD138_Cytof_060314.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DL-101_Purified_CD138_Cytof_060314.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
U266 (blue) and Jurkat (red) cells stained with 145Nd-anti-CD138 (DL-101). Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
352311 | 100 µg | £109 |
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human SDC1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2
Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1. - Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC)
- Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed
- RRID
-
AB_2563719 (BioLegend Cat. No. 352311)
Antigen Details
- Structure
- 100-200 kD type I integral transmembrane glycoprotein
- Distribution
-
Plasma cells, pre-B cells, epithelial cells, endothelial cells
- Function
- Adhesion, control cell morphology, regulate cell growth
- Ligand/Receptor
- FGFb, collagen, fibronectin
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Plasma cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication. - Gene ID
- 6382 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD138 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD138 (Syndecan-1) | DL-101 | FC,IHC-P |
FITC anti-human CD138 (Syndecan-1) | DL-101 | FC |
PE anti-human CD138 (Syndecan-1) | DL-101 | FC |
APC anti-human CD138 (Syndecan-1) | DL-101 | FC |
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1) | DL-101 | FC |
Purified anti-human CD138 (Syndecan-1) (Maxpar® Ready) | DL-101 | FC,CyTOF® |
PE/Cyanine7 anti-human CD138 (Syndecan-1) | DL-101 | FC |
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1) | DL-101 | FC |
Alexa Fluor® 647 anti-human CD138 (Syndecan-1) | DL-101 | FC |
APC/Fire™ 750 anti-human CD138 (Syndecan-1) | DL-101 | FC |
Biotin anti-human CD138 (Syndecan-1) | DL-101 | FC |
Alexa Fluor® 594 anti-human CD138 (Syndecan-1) | DL-101 | IHC-P |
TotalSeq™-A0831 anti-human CD138 (Syndecan-1) | DL-101 | PG |
TotalSeq™-C0831 anti-human CD138 (Syndecan-1) | DL-101 | PG |
TotalSeq™-B0831 anti-human CD138 (Syndecan-1) Antibody | DL-101 | PG |
TotalSeq™-Bn0831 anti-human CD138 (Syndecan-1) | DL-101 | SB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with purified CD138... Human paraffin-embedded colon tissue slices were prepared wi... -
FITC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
PE anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
APC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
Purified anti-human CD138 (Syndecan-1) (Maxpar® Ready)
U266 (blue) and Jurkat (red) cells stained with 145Nd-anti-C... -
PE/Cyanine7 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
APC/Fire™ 750 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
Biotin anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone D... -
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)
Human paraffin-embedded colon tissue slices were prepared wi... -
TotalSeq™-A0831 anti-human CD138 (Syndecan-1)
-
TotalSeq™-C0831 anti-human CD138 (Syndecan-1)
-
TotalSeq™-B0831 anti-human CD138 (Syndecan-1) Antibody
-
TotalSeq™-Bn0831 anti-human CD138 (Syndecan-1)
Follow Us