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Purified anti-human CD140b (PDGFRβ) Recombinant Antibody

Pricing & Availability
Clone
QA20A36 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet-derived growth factor receptor, beta polypeptide, PDGFR1, PDGFRβ, PDGFRb, PDGF receptor beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A36_PURE_CD140b_Recom_Antibody_041822
Human PDGFRB transfected cells stained with purified anti-human CD140b (clone QA20A36) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • QA20A36_PURE_CD140b_Recom_Antibody_041822
    Human PDGFRB transfected cells stained with purified anti-human CD140b (clone QA20A36) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
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383002 100 µg £81
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Description

CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety of mesenchymal-derived cells and is preferentially expressed on some tumors such as medulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cell proliferation. CD140b has been shown to interact with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2, Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3 and nexin sorting molecules. CD140b has been implicated in several disease states including atherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927818 (BioLegend Cat. No. 383002)

Antigen Details

Structure
Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR1 and PD.
Distribution

Widely expressed on a variety of mesenchymal-derived cells. Preferentially expressed in medulloblastoma.

Function
Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Has been implicated in atherogenesis and oncogenesis.
Interaction
Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin β3 and nexin sorting molecules.
Ligand/Receptor
Binds to B-chain containing PDGF molecules as well as protease-activated PDGF-C
Antigen References
  1. Claesson-Welsh L, et al. 1988. Mol Cell Biol. 8:3476-86.
  2. Gronwald RG, et al. 1988. Proc Natl Acad Sci U S A. 85:3435-9.
  3. Gilbertson DG, et al. 2001. J Biol Chem. 276:27406-14.
  4. Seifert RA, et al. 1989. J Biol Chem. 264:8771-8.
  5. Kanakaraj P, et al. 1991. Biochemistry. 30:1761-7.
Gene ID
5159 View all products for this Gene ID
UniProt
View information about CD140b on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/18/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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