Purified anti-human CD152 (CTLA-4) Recombinant Antibody

Pricing & Availability
Clone
QA20B10 (See other available formats)
Regulatory Status
RUO
Other Names
CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20B10_-Purified_-CD152_Antibody_030323
Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, fixed, permeabilized, and intracellularly stained with purified anti-human CD152 (CTLA-4) recombinant (clone QA20B10) (left), or purified mouse IgG1, κ isotype control (right), followed by PE goat anti-mouse IgG. Cells were constained with anti-human CD3 APC.
  • QA20B10_-Purified_-CD152_Antibody_030323
    Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, fixed, permeabilized, and intracellularly stained with purified anti-human CD152 (CTLA-4) recombinant (clone QA20B10) (left), or purified mouse IgG1, κ isotype control (right), followed by PE goat anti-mouse IgG. Cells were constained with anti-human CD3 APC.
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385402 100 µg £125
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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4  is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA20B10 can completely block the binding of parental clone to target cells.

RRID
AB_2936635 (BioLegend Cat. No. 385402)

Antigen Details

Structure
Ig superfamily and 33 kD
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Interaction
Antigen presenting cells
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86)
Antigen References
  1. Kuiper HM, et al. 1995. J Immunol. 155:1776-83.
  2. Pistillo MP, et al. 2003. Blood. 101:202-9.
  3. Tan PH, et al. 2005. Blood. 106:2936-43.
  4. Steiner K, et al. 2001. Clin Exp Immunol. 126:143-50.
Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org
Go To Top Version: 1    Revision Date: 03/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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