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- Clone
- QA20B10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, fixed, permeabilized, and intracellularly stained with purified anti-human CD152 (CTLA-4) recombinant (clone QA20B10) (left), or purified mouse IgG1, κ isotype control (right), followed by PE goat anti-mouse IgG. Cells were constained with anti-human CD3 APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 385402 | 100 µg | £125 | ||||
CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone QA20B10 can completely block the binding of parental clone to target cells.
- RRID
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AB_2936635 (BioLegend Cat. No. 385402)
Antigen Details
- Structure
- Ig superfamily and 33 kD
- Distribution
-
Activated T cells and B cells
- Function
- Negative regulator of T cell activation
- Interaction
- Antigen presenting cells
- Ligand/Receptor
- B7-1 (CD80), B7-2 (CD86)
- Antigen References
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- Kuiper HM, et al. 1995. J Immunol. 155:1776-83.
- Pistillo MP, et al. 2003. Blood. 101:202-9.
- Tan PH, et al. 2005. Blood. 106:2936-43.
- Steiner K, et al. 2001. Clin Exp Immunol. 126:143-50.
- Gene ID
- 1493 View all products for this Gene ID
- UniProt
- View information about CD152 on UniProt.org
Other Formats
View All CD152 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD152 (CTLA-4) Recombinant Antibody | QA20B10 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD152 (CTLA-4) Recombinant Antibody
Human peripheral blood mononuclear cells were stimulated wit...
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