Purified anti-human CD154 Maxpar Ready Antibody anti-CD154

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Clone: 24-31 (See other available formats)
Regulatory Status: RUO
Other Names: CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs were incubated for 6 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 170Er-anti-CD3 (UCHT1) and 168Er-anti-CD154 [CD40L] (24-31). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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310835

100 µg

£157

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Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3 and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563722 (BioLegend Cat. No. 310835)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD154 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD154	24-31	FC,IHC-F
FITC anti-human CD154	24-31	FC,IHC-F
PE anti-human CD154	24-31	FC
PE/Cyanine5 anti-human CD154	24-31	FC
APC anti-human CD154	24-31	FC
Biotin anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 488 anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 647 anti-human CD154	24-31	FC,IHC-F
Pacific Blue™ anti-human CD154	24-31	FC
Brilliant Violet 785™ anti-human CD154	24-31	FC
APC/Cyanine7 anti-human CD154	24-31	FC
Brilliant Violet 421™ anti-human CD154	24-31	FC,IHC-F
Brilliant Violet 605™ anti-human CD154	24-31	FC
Ultra-LEAF™ Purified anti-human CD154	24-31	FC,Block,IHC-F
Brilliant Violet 510™ anti-human CD154	24-31	FC,IHC-F
PE/Cyanine7 anti-human CD154	24-31	FC
PerCP/Cyanine5.5 anti-human CD154	24-31	FC
Brilliant Violet 711™ anti-human CD154	24-31	FC
Purified anti-human CD154 (Maxpar® Ready)	24-31	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD154	24-31	FC
TotalSeq™-A0032 anti-human CD154	24-31	PG
Alexa Fluor® 700 anti-human CD154	24-31	FC
APC/Fire™ 750 anti-human CD154	24-31	FC
TotalSeq™-C0032 anti-human CD154	24-31	PG
TotalSeq™-B0032 anti-human CD154	24-31	PG
TotalSeq™-D0032 anti-human CD154	24-31	PG
PE/Fire™ 640 anti-human CD154	24-31	FC
PE/Fire™ 810 anti-human CD154	24-31	FC
PerCP/Fire™ 780 anti-human CD154	24-31	FC
PE/Fire™ 700 anti-human CD154	24-31	FC
Spark Red™ 718 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Brilliant Violet 650™ anti-human CD154	24-31	FC
Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™)	24-31	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.