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Purified anti-human CD202b (Tie2/Tek) Antibody

Pricing & Availability
Clone
33.1 (Ab33) (See other available formats)
Regulatory Status
RUO
Other Names
Tie2, TEK
Isotype
Mouse IgG1, κ
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Product Citations
publications
AB33_Pure_070808.jpg
Human endothelial cells (HUVEC) stained with purified AB33, followed by biotinylated anti-mouse IgG and Sav-PE
  • AB33_Pure_070808.jpg
    Human endothelial cells (HUVEC) stained with purified AB33, followed by biotinylated anti-mouse IgG and Sav-PE
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334202 100 µg £81
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Description

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of human Tie2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, IHC-F , ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Peters KG, et al. 1998. Br. J. Cancer. 77:51.
  2. Wong AL, et al. 1997. Circ. Res. 81:567.
  3. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
RRID
AB_1134251 (BioLegend Cat. No. 334202)

Antigen Details

Structure
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution

Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes

Function
Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand/Receptor
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1.De Palma M, et al. 2005. Cancer Cell. 8:211
2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168
3. Hsu HC, et al. 2000. Blood. 96:3757
4. Arai F, et al. 2004. Cell. 118:149

Gene ID
7010 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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