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Purified anti-human CD207 (Langerin) Antibody

Pricing & Availability
Clone
10E2 (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4K
Isotype
Mouse IgG1, κ
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Product Citations
publications
10E2_PE_1_060311
Monocyte-derived Langerhans cells were stimulated with recombinant human GM-CSF, TGF-β and IL-4 for 3 days, followed by an additional 3-day culture with rhGM-CSF and TGF-β. They were then stained with CD1a APC and CD207 (clone 10E2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 10E2_PE_1_060311
    Monocyte-derived Langerhans cells were stimulated with recombinant human GM-CSF, TGF-β and IL-4 for 3 days, followed by an additional 3-day culture with rhGM-CSF and TGF-β. They were then stained with CD1a APC and CD207 (clone 10E2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 10E2_PE_2_060311
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352202 100 µg £157
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Description

CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Primary human Langerhans cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Witte LD, et al. 2007. Nat. Med. 13:367. (Block)
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
RRID
AB_10898115 (BioLegend Cat. No. 352202)

Antigen Details

Structure
40 kD glycoprotein
Distribution

Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells

Function
Binding and processing antigen; capturing, internalizing and degrading HIV
Ligand/Receptor
Antigen containing mannose, N-acetyl glucosamine, and fucose
Cell Type
Dendritic cells, Langerhans cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Valladeau J, et al. 2000. Immunity 12:71.
2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317.
3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701.
4. Witte LD, et al. 2007. Nat. Med. 13:367.

Gene ID
50489 View all products for this Gene ID
UniProt
View information about CD207 on UniProt.org
Go To Top Version: 3    Revision Date: 08/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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