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Purified anti-human CD209/CD299 (DC-SIGN/L-SIGN) Antibody

Pricing & Availability
Clone
A20120F (See other available formats)
Regulatory Status
RUO
Other Names
For CD209: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, For CD299: LSIGN, CD209L, L-SIGN, DCSIGNR, HP10347, DC-SIGN2, DC-SIGNR
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20120F_PURE_CD209-CD299_Antibody_052322
Human monocyte-derived dendritic cells were stained with purified anti-human CD209/CD299 (DC-SIGN/L-SIGN) (clone A20120F) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by PE goat anti-rat IgG.
  • A20120F_PURE_CD209-CD299_Antibody_052322
    Human monocyte-derived dendritic cells were stained with purified anti-human CD209/CD299 (DC-SIGN/L-SIGN) (clone A20120F) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by PE goat anti-rat IgG.
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380502 100 µg £177
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Description

CD209 (DC-SIGN) is a C-type lectin that has a high affinity for the ICAM3 molecule. It binds various microorganisms by recognizing high mannose-containing glycoproteins on their envelopes. It especially functions as a receptor for several viruses such as HIV and Hepatitis C. CD299 (L-SIGN) is a transmembrane receptor and is referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD209 protein extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A20120F can completely block the binding of clone 14E3G7 to target cells. It does not affect the binding of clone A20120B, clone 9E9A8 and clone DCS-8C1.

RRID
AB_2924572 (BioLegend Cat. No. 380502)

Antigen Details

Structure
DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
Distribution

Dendritic cells, endothelial cells, macrophages

Function
CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
Ligand/Receptor
CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References
  1. Fauci AS. 1996. Nature. 384:529-34.
  2. Yokoyama-Kobayashi M, et al. 1999. Gene. 228:161-7.
  3. Soilleux EJ, et al. 2000. J Immunol. 165:2937-42.
  4. Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670-5.
  5. Bashirova AA, et al. 2001. J Exp Med. 193:671-8.
  6. Lai WK, et al. 2006. Am J Pathol. 169:20-8.
Gene ID
30835 View all products for this Gene ID 10332 View all products for this Gene ID
UniProt
View information about CD209/CD299 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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