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Purified anti-human CD228 (MFI2, MTF1) Antibody

Pricing & Availability
Clone
SHM72 (See other available formats)
Regulatory Status
RUO
Other Names
MFI2, MAP97, MTF1, Melanotransferrin, Membrane-bound transferrin-like protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SHM72_Purified_CD228_Antibody_FC_072814.jpg
Human malignant melanoma cell line, A375, was stained with purified CD228 (clone SHM72, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • SHM72_Purified_CD228_Antibody_FC_072814.jpg
    Human malignant melanoma cell line, A375, was stained with purified CD228 (clone SHM72, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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363101 100 µg £182
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Description

CD228 is a cell-surface sialoglycoprotein found on melanoma cells that consists of a cell membrane, lipid-anchor and GPI-anchor domains. It is involved in iron uptake and shares sequence similarity and iron-binding properties that are found in members of the transferrin superfamily. CD228 is internalized before being recycled back to the cell membrane and is also capable of binding zinc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Mazahreh R, et al. 2023. Mol Cancer Ther. 421:22. PubMed
RRID
AB_2564116 (BioLegend Cat. No. 363101)

Antigen Details

Structure
Cell membrane, lipid-anchor, GPI-anchor, 80 kD
Distribution

Melanoma cells

Function
Involved in iron uptake
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Farnaud S, et al. 2008. Int. J. Biochem. Cell Biol. 40:2739.
2. Sekyere EO, et al. 2005. Biochim. Biophys. Acta. 1722:131.
3. Dunn LL, et al. 2006. Carcinogenesis. 27:2157.
4. Suryo Rahmanto Y, et al. 2007. Carcinogenesis. 28:2172.

Gene ID
4241 View all products for this Gene ID
UniProt
View information about CD228 on UniProt.org
Go To Top Version: 2    Revision Date: 08/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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