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Purified anti-human CD243 (MDR-1) Antibody

Pricing & Availability
Clone
UIC2 (See other available formats)
Regulatory Status
RUO
Other Names
Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
UIC2_PE_051010
Human neuroblastoma cell line SK-N-FI stained with purified UIC2 conjugated with PE
  • UIC2_PE_051010
    Human neuroblastoma cell line SK-N-FI stained with purified UIC2 conjugated with PE
  • UIC2_PURE_CD243_Antibody_IHC_Validated_092617
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Purified anti-CD243 (Clone UIC2) overnight at 4°C. On the next day, tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG antibody (Clone POLY4053, red) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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348602 100 µg £145
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Description

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH 3T3 cells transfected with human MDR-1 cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume.For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking the efflux of fluorescent dyes1,2, immunoprecipitation1, and immunohistochemical staining of tissue sections of squamous cell carcinoma3.

Application References

(PubMed link indicates BioLegend citation)
  1. Mechetner EB and Roninson IB. 1992. Proc. Natl. Acad. Sci. USA 13:5824. (Block, IP)
  2. Chaudhary PM, et al. 1992. Blood 80:2735. (Block)
  3. Kelley DJ, et al. 1993. Arch. Otolaryngol. Head Neck Surg. 119:411. (IHC-P)
  4. Goda K, et al. 2007. J. Pharmacol. Exp. Ther. 320:81. (Block)
RRID
AB_10576371 (BioLegend Cat. No. 348602)

Antigen Details

Structure
Member of ABC transporters family, MW ~170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution

Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.

Function
Transport various molecules across cellular membranes. Involved in multidrug resistance.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Dulucq S, et al. 2008. Blood 112:2024. 
2. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546. 
3. Andreadis C, et al. 2007. Blood 109:3409.

Gene ID
5243 View all products for this Gene ID
UniProt
View information about CD243 on UniProt.org
Go To Top Version: 2    Revision Date: 09/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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