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Purified anti-human CD262 (DR5, TRAIL-R2) Antibody

Pricing & Availability
Clone
A21121I (See other available formats)
Regulatory Status
RUO
Other Names
TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A21121I_Pure_CD262_Antibody_1_061924
U937 cells were stained with purified anti-human CD262 (DR5, TRAIL-R2) (clone A21121I) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • A21121I_Pure_CD262_Antibody_1_061924
    U937 cells were stained with purified anti-human CD262 (DR5, TRAIL-R2) (clone A21121I) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • A21121I_Pure_CD262_Antibody_2_061924
    Human peripheral blood monocytes were stained with purified anti-human CD262 (DR5, TRAIL-R2) (clone A21121I) (left) or purified mouse IgG2a, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were then stained with anti-human CD14 (clone HCD14) APC.
  • A21121I_Pure_CD262_Antibody_3_061924
    ICC staining of purified anti-human CD262 (DR5, TRAIL-R2) (clone A21121I) (panel A) on HCC827, a human lung adenocarcinoma cell line. The cells were fixed with 2% PFA and blocked with 5% FBS. The cells were then stained with 5 µg/mL of the purified anti-human TNFRSF10B antibody (left) or purified mouse IgG2a, κ isotype control (panel B) overnight at 4°C followed by goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 for an hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
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390704 100 µg £137
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Description

DR5, also known as TRAIL-R2, TRICK2, KILLER, and CD262, is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B). It binds the cytotoxic ligand, TRAIL, and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TNFRSF10B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A21121I does not block the staining of reference clone DJR2-4 (7-8)

Recombinant protein completely blocks the staining of test clone A21121I.

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution

Broadly expressed on normal and tumor cells

Function
Induces apoptosis
Ligand/Receptor
TRAIL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. MacFarlane M, et al. 1997. J Biol Chem. 272:25417-20.
  2. Walczak H, et al. 1997. EMBO J. 16:5386-97.
  3. Shigeno M, et al. 2003. Oncogene. 22:1653-62.
  4. LaVallee TM, et al. 2003. Cancer Res. 63:468-75.
  5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759-63.
Gene ID
8795 View all products for this Gene ID
UniProt
View information about CD262 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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