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Purified anti-human CD276 (B7-H3) Antibody

Pricing & Availability
Clone
W5C4 (See other available formats)
Regulatory Status
RUO
Other Names
4Ig-B7-H3, B7RP2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W5C4_PURE_CD276_Antibody_122321
Human monocyte derived dendritic cells were stained with purified anti-human CD276 (clone W5C4) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
  • W5C4_PURE_CD276_Antibody_122321
    Human monocyte derived dendritic cells were stained with purified anti-human CD276 (clone W5C4) (filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
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377002 100 µg £233
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Description

B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human retinoblastoma cell line, WERI-RB-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tryfonidou M, et al. 2014. Cytometry A. 85:379-81. (FC)
  2. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:e50. (FC)
  3. Buhring H J, et al. 2007. Ann N Y Acad Sci. 1106:262-71. (FC)
RRID
AB_2910432 (BioLegend Cat. No. 377002)

Antigen Details

Structure
Type I transmembrane protein with extracellular variable-type immunoglobulin domain and intracellular domain
Distribution

Professional APCs, including B cells, dendritic cells, macrophages, and mesenchymal stem cells

Ligand/Receptor
Trem like transcript 2 protein (TLT-2)
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Immuno-Oncology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Sun M, et al. 2002. J. Immunology. 168:6294-7.
  2. Xu J, et al. 2006. Cell Mol Immunol. 3:235-40.
  3. Sugawara E, et al. 2020. Autophagy. 16:946-955.
Gene ID
80381 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/23/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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