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Purified anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
NAT105 (See other available formats)
Regulatory Status
RUO
Other Names
PD1, PDCD1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_NAT105_PURE_CD279_Antibody_FC_072915
Human peripheral blood lymphocytes were stained with purified CD279 (clone NAT105), followed by anti-mouse IgG PE. The cells were then stained with anti-CD3 APC.
  • A_NAT105_PURE_CD279_Antibody_FC_072915
    Human peripheral blood lymphocytes were stained with purified CD279 (clone NAT105), followed by anti-mouse IgG PE. The cells were then stained with anti-CD3 APC.
  • B_NAT105_PURE_CD279_Antibody_IHCP_08242017
    Tissue imaged here is the lymphoid nodule/germinal center of a human tonsil. CD279 positive staining is prevalent within the nodule/germinal center. The antibody was used at 10 μg/ml here. Images shown at 10x and 40x magnification (inset).
  • C_NAT105_PURE_CD279_Antibody_IHCP_113017
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffered 1x (1.0M, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified CD279 (clone NAT105) antibody overnight at 4°C. On the next day, the tissue was washed twice with 1x PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053, red) for an hour. Nuclei were counterstained with DAPI (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • D_NAT105_PURE_CD279_Antibody_WB_082417
    Western blot analysis of Jurkat (lane 1), PBMC untreated (lane 2) and PBMC activated with CD3 (clone OKT3) and CD28 (clone 28.2) antibodies for two days (lane 3) using anti-CD279 antibody (clone NAT105). Anti-β-actin antibody (poly6221) was used as a loading control.
  • E_NAT105_PURE_CD279_Antibody_FC_110215
    PHA-stimulated (three days) human PBMC were stained with purified CD279 (clone NAT105) (filled histogram) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.
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367402 100 µg £77
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry of paraffin-embedded tissue, a concentration range of 0.1 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue Sections: Paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R. Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 24 hours at 2°C - 4 °C.
Positive Control: Human tonsil and spleen.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
Product Citations
  1. Sullivan KMC, et al. 2023. PLoS One. 18:e0286724. PubMed
  2. Kim JH, et al. 2020. Foods. 9:00. PubMed
  3. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
RRID
AB_2565782 (BioLegend Cat. No. 367402)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/02/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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