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Purified anti-human CD279 (PD-1) Recombinant Antibody

Pricing & Availability
Clone
QA19A98 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A98_PURE_CD279_Antibody_020821
PHA-stimulated (3 days) human peripheral blood mononuclear lymphocytes were stained with purified anti-human CD279 (PD-1) recombinant (clone QA19A98) (left) or mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then co-stained with CD3 APC.
  • QA19A98_PURE_CD279_Antibody_020821
    PHA-stimulated (3 days) human peripheral blood mononuclear lymphocytes were stained with purified anti-human CD279 (PD-1) recombinant (clone QA19A98) (left) or mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then co-stained with CD3 APC.
  • QA19A98_PURE_CD279_Antibody_2_121322
    IHC staining of purified anti-human CD279 (clone QA19A98) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20 (Cat. No. 925501) the tissue was incubated with blocking buffer. Tissue was then stained without (panel A) or with 10 µg/mL of primary antibody (panel B) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801) and images were captured with 10X (panels A and B) and 40X (panels C and D) objectives. Scale bar: 50 µm
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322102 100 µg £61
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Description

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Human CD279
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA19A98 antibody is able to completely block binding of clone A17188B on target cells.

This recombinant antibody clone is derived from clone A17188B.

RRID
AB_2890754 (BioLegend Cat. No. 322102)

Antigen Details

Structure
55 kD type 1 transmembrane protein
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells

Function
Signaling, co-stimulation, co-inhibition
Interaction
SHP-1 and SHP-2
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 3    Revision Date: 12/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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