Purified anti-human CD303 BDCA-2 Maxpar Ready Antibody anti-CD303

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Clone: 201A (See other available formats)
Regulatory Status: RUO
Other Names: CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human PBMCs stained with 151Eu-anti-CD123 (6H6) and 153Eu-anti-CD303 (201A). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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354215

100 µg

£177

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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563739 (BioLegend Cat. No. 354215)

Antigen Details

Structure: Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution: Plasmacytoid dendritic cells
Function: Antigen capture, inhibition of IFN α/β production
Cell Type: Dendritic cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.
Gene ID: 170482 View all products for this Gene ID
UniProt: View information about CD303 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD303 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD303 (BDCA-2)	201A	FC
Purified anti-human CD303 (BDCA-2)	201A	FC
PE anti-human CD303 (BDCA-2)	201A	FC
FITC anti-human CD303 (BDCA-2)	201A	FC
PerCP/Cyanine5.5 anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 421™ anti-human CD303 (BDCA-2)	201A	FC
PE/Cyanine7 anti-human CD303 (BDCA-2)	201A	FC
Purified anti-human CD303 (BDCA-2) (Maxpar® Ready)	201A	FC,CyTOF®
Alexa Fluor® 647 anti-human CD303 (BDCA-2)	201A	FC
Biotin anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 785™ anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 605™ anti-human CD303 (BDCA-2)	201A	FC
PE/Dazzle™ 594 anti-human CD303 (BDCA-2)	201A	FC
Alexa Fluor® 700 anti-human CD303 (BDCA-2)	201A	FC
Alexa Fluor® 488 anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 510™ anti-human CD303 (BDCA-2)	201A	FC
Brilliant Violet 711™ anti-human CD303 (BDCA-2)	201A	FC
APC/Fire™ 750 anti-human CD303 (BDCA-2)	201A	FC
APC/Cyanine7 anti-human CD303 (BDCA-2)	201A	FC
TotalSeq™-A0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-C0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-B0370 anti-human CD303 (BDCA-2)	201A	PG
TotalSeq™-D0370 anti-human CD303 (BDCA-2)	201A	PG
Spark Blue™ 550 anti-human CD303 (BDCA-2) (Flexi-Fluor™)	201A	FC
Spark Red™ 718 anti-human CD303 (BDCA-2) (Flexi-Fluor™)	201A	FC
APC/Fire™ 810 anti-human CD303 (BDCA-2)	201A	FC
Spark Violet™ 538 anti-human CD303 (BDCA-2)	201A	FC
PerCP/Fire™ 806 anti-human CD303 (BDCA-2)	201A	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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