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Purified anti-human CD304 Antibody

Pricing & Availability
Clone
14H4 (See other available formats)
Regulatory Status
RUO
Other Names
Neuropilin 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
14H4_050912
Whole cell extracts of HUVEC cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti CD304 (clone 14H4) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 14H4_050912
    Whole cell extracts of HUVEC cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti CD304 (clone 14H4) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 14H4_PURE_CD304_Antibody_WB_2_120117
    Total cell lysates (15 µg protein) from PC3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to a nitrocellulose membrane, and probed with serial dilutions of purified anti-CD304 antibody (clone 14H4), 1:1000 (0.5 µg/mL, lane 1), 1:500 (1 µg/mL, lane 2) and 1:250 (2 µg/mL, lane 3). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated goat anti-mouse-IgG secondary antibody for the anti-CD304 (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. Non-specific bands can be detected at high antibody concentrations.
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652302 100 µg £221
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Description

The membrane isoform of CD304 is a receptor that regulates angiogenesis and organogenesis in response to growth factors of the VEGF family. CD304 forms a complex with VEGFR2/KDR, resulting in increased cell migration and chemotaxis. In the nervous system, CD304 complexed with plexin mediates Sema-3A signaling and may be involved in axon guidance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
293E expressed human CD304 recombinant protein, b isoform
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel (1 µg/ml). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_11203372 (BioLegend Cat. No. 652302)

Antigen Details

Structure
Single-pass type I membrane protein, 103 kD. Contains two CUB motifs, domains similar to coagulation factors V and VIII, and a MAM domain in the extracellular domain. Isoform b is a soluble form.
Distribution

Membrane, Secreted (isoform b).

Function
CD304 is a VEGF co-receptor involved in axon guidance, angiogenesis. and development of cardiovascular system. The secreted isoform inhibits binding of VEGF-165 to cells.
Ligand/Receptor
CD304 is a receptor for members of VEGF family and pro-angiogenesis cytokines, such as VEGF-A165, VEGF-B, VEGF-E, and PIGF-2.
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Soker S, et al. 1998. Cell 92:735.
2. Shintani Y, et al. 2006. EMBO J. 25:3045.
3. He Z, et al. 1997. Cell 90:739.
4. Gagnon ML, et al. 2000. P. Natl. Acad. Sci. USA 97:2573.
5. Glinka Y, et al. 2011. Carcinogenesis 32:613.

Gene ID
8829 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org
Go To Top Version: 2    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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