- Clone
- A16085I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- VEGFR2, KDR
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A16085I_PURE_CD309_Antibody_1_IHC_012122 A16085I_PURE_CD309_Antibody_1_IHC_012122](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A16085I_PURE_CD309_Antibody_1_IHC_012122.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD309/VEGFR2 (clone A16085I) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. -
Human paraffin-embedded breast cancer section slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD309/VEGFR2 (clone A16085I) purified at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. -
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD309/VEGFR2 (clone A16085I) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. -
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD309/VEGFR2 (clone A16085I) and anti-human PNAD (clone MECA-79) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for two hours at room temperature. The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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374002 | 100 µg | £157 |
CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human VEGFR2-Fc Chimera
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2687130 (BioLegend Cat. No. 374002)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
- Distribution
-
Endothelial cells, embryonic tissues
- Function
- VEGFR2 regulates angiogenesis as well as vascular development and permeability
- Interaction
- VEGF-A, SHC2, NPR1, NCK1
- Ligand/Receptor
- VEGF-A, VEGF-C, and VEGF-D splice isoforms
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. DiSalvo J, et al. 1995. J. Biol. Chem. 270:7717.
2. McColl BK, et al. 2003. J. Exp. Med. 198:863.
3. Albuquerque RJ, et al. 2009. Nat. Med. 15:1023.
4. Guangqi E, et al. 2012. J. Biol. Chem. 287:3029.
5. Koch S, and Claesson-Welsh 2012. Cold Spring Harb Perspect Med 2:a006502.
6. Munaut C, et al. 2012. Plos One 7:e33475.
7. Wehland M, et al. 2013. Int. J. Mol. Sci. 14:9338. - Gene ID
- 3791 View all products for this Gene ID
- UniProt
- View information about CD309 on UniProt.org
Other Formats
View All CD309 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD309 (VEGFR2) | A16085I | IHC-P |
Alexa Fluor® 594 anti-human CD309 (VEGFR2) | A16085I | IHC-P |
Spark YG™ 570 anti-human CD309 (VEGFR2) | A16085I | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD309 (VEGFR2)
Human paraffin-embedded placenta tissue slices were prepared... Human paraffin-embedded breast cancer section slices were pr... Human paraffin-embedded placenta tissue slices were prepared... Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD309 (VEGFR2)
Human paraffin-embedded placenta tissue slices were prepared... -
Spark YG™ 570 anti-human CD309 (VEGFR2)
Human paraffin-embedded placenta tissue slices were prepared...
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