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Purified anti-human CD312 (EMR2) Antibody

Pricing & Availability
Clone
W15101A (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal growth factor module-containing mucin-like receptor 2, EMR2, AGRE2, ADGRE2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15101A_PURE_CD312_Antibody_021417
Human peripheral blood monocytes were stained with purified CD312 (clone W15101A, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG-PE.
  • W15101A_PURE_CD312_Antibody_021417
    Human peripheral blood monocytes were stained with purified CD312 (clone W15101A, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG-PE.
  • W15101A_PURE_CD312_Antibody_IHC_033017
    Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD312 (clone W15101A) and 10 µg/ml of Brilliant Violet 421™ anti-human CD19 (clone SJ25C1) (blue) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. The image was captured by 10X objective.
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373102 100 µg £209
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Description

CD312, also known as Epidermal growth factor module-containing mucin-like receptor 2 (EMR2), is a 90 kD type I protein, a member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family. Its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site, while the C-terminal region consists of a seven-pass transmembrane domain. CD312 is expressed by myeloid cells, dendritic cells, and activated lymphocytes. CD312 contributes to the interaction of macrophages, dendritic cells and activated T cells with B cells; it is involved in neutrophil chemotaxis, degranulation and adhesion, and release of inflammatory cytokines by macrophages. The ligands of CD312 are the glycosaminoglycans (GAG) chondroitin sulphate, and dermatan sulphate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein fragment containing the first extracellular region of human EMR2 (24-540 a.a.).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650810 (BioLegend Cat. No. 373102)

Antigen Details

Structure
Member of the Epidermal Growth Factor-seven transmembrane (EGF-TM7) family, type I protein, its extracellular region consists of five tandem EGF-like adhesion domains and a mucin-like stalk domain containing a G-protein proteolytic site; the C-terminal re
Distribution

Expressed by myeloid cells, dendritic cells, and activated lymphocytes.

Function
Involved in neutrophil chemotaxis, degranulation and adhesion. Promotes the release of inflammatory cytokines by macrophages. Contributes in the interaction of macrophages, dendritic cells and activated T cells with B cells.
Ligand/Receptor
Chondroitin sulphate, dermatan sulphate.
Cell Type
Dendritic cells, Lymphocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Boyden SE, et al. 2016. N. Engl. J. Med. 374:656.
2. Lewis SM, et al. 2015. Clin. Exp. Immunol. 182:184.
3. Huang YS, et al. 2012. Mol. Cell Biol. 32:1408.
4. Rutkowski MJ, et al. 2011. J. Neurooncol. 105:165.
5. van Eijk M, et al. 2010. Immunol. Lett. 129:64.  

Gene ID
30817 View all products for this Gene ID
UniProt
View information about CD312 on UniProt.org
Go To Top Version: 1    Revision Date: 02/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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