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Purified anti-human CD33 Antibody

Pricing & Availability
Clone
WM53 (See other available formats)
Regulatory Status
RUO
Workshop
IV M-505
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
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Product Citations
publications
WM53_Purified_062507
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
  • WM53_Purified_062507
    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
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303402 100 µg £70
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myeloid leukaemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
  3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
  4. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  2. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  3. Consonni FM, et al. 2023. Front Immunol. 14:1168455. PubMed
  4. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  5. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  6. Wen J, et al. 2020. J Pathol Clin Res. 0.36875. PubMed
  7. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  8. Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
  9. Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
  10. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  11. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  12. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  13. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  14. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  15. Henrick BM, et al. 2021. Cell. . PubMed
  16. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  17. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  18. Hou Z, et al. 2020. Oncol Lett. 19:3982. PubMed
  19. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  20. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_314346 (BioLegend Cat. No. 303402)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 09/06/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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