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Purified anti-human CD366 (Tim-3) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
F38-2E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
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Product Citations
publications
F38-2E2_Purified_Tim-3_Cytof_1_061114.jpg
Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom). Cells were then stained with 145Nd-anti-CD4 (RPA-T4) and 153Eu-anti-human CD366 (Tim-3, clone F38-2E2). CD3+ T cells are displayed in the analysis. Data provided by DVS Sciences.
  • F38-2E2_Purified_Tim-3_Cytof_1_061114.jpg
    Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom). Cells were then stained with 145Nd-anti-CD4 (RPA-T4) and 153Eu-anti-human CD366 (Tim-3, clone F38-2E2). CD3+ T cells are displayed in the analysis. Data provided by DVS Sciences.
  • F38-2E2_Purified_Tim-3_Cytof_2_061114.jpg
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345019 100 µg £177
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Tim-3 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
  2. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  3. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  4. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  5. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
RRID
AB_2563790 (BioLegend Cat. No. 345019)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD366 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD366 (Tim-3) F38-2E2 FC
PE anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 421™ anti-human CD366 (Tim-3) F38-2E2 FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC anti-human CD366 (Tim-3) F38-2E2 FC
PE/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 605™ anti-human CD366 (Tim-3) F38-2E2 FC
FITC anti-human CD366 (Tim-3) F38-2E2 FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready) F38-2E2 FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3) F38-2E2 FC
APC/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 785™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 650™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 510™ anti-human CD366 (Tim-3) F38-2E2 FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3) F38-2E2 FC
GoInVivo™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3) F38-2E2 FC
Pacific Blue™ anti-human CD366 (Tim-3) F38-2E2 FC
Biotin anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-A0169 anti-human CD366 (Tim-3) F38-2E2 PG
TotalSeq™-C0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Cyanine5 anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-B0169 anti-human CD366 (Tim-3) F38-2E2 PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody F38-2E2 FC
TotalSeq™-D0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Fire™ 810 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 640 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 700 anti-human CD366 (Tim-3) F38-2E2 FC
Go To Top Version: 4    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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