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Purified anti-human CD38 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
HIT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 155
Other Names
T10, ADP-ribosyl cyclase
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIT2_Purified_CD38_CyTOF_112113
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 167Er-anti-CD38 (HIT2). Data provided by DVS Sciences.
  • HIT2_Purified_CD38_CyTOF_112113
    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 167Er-anti-CD38 (HIT2). Data provided by DVS Sciences.
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303535 100 µg £89
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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Horse, Cow
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373.
  3. Esser M, et al. 2001. J. Virol. 75:6173.
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044.
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
  6. van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
  7. Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
  8. Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  2. Labanieh L, et al. 2022. Cell. 185:1745. PubMed
  3. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  4. NULL, et al. 2022. Cell. 185:916. PubMed
RRID
AB_2562819 (BioLegend Cat. No. 303535)

Antigen Details

Structure
ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution

T cells, B cells, NK, myeloid, plasma, and dendritic cells

Function
Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.

Gene ID
952 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD38 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD38 HIT2 FC
FITC anti-human CD38 HIT2 FC
PE anti-human CD38 HIT2 FC
PE/Cyanine5 anti-human CD38 HIT2 FC
Purified anti-human CD38 HIT2 FC,CyTOF®,IHC-F
Alexa Fluor® 488 anti-human CD38 HIT2 FC
Alexa Fluor® 647 anti-human CD38 HIT2 FC
PE/Cyanine7 anti-human CD38 HIT2 FC
Biotin anti-human CD38 HIT2 FC
PerCP anti-human CD38 HIT2 FC
PerCP/Cyanine5.5 anti-human CD38 HIT2 FC
Alexa Fluor® 700 anti-human CD38 HIT2 FC,SB
Brilliant Violet 421™ anti-human CD38 HIT2 FC
Brilliant Violet 711™ anti-human CD38 HIT2 FC
Brilliant Violet 785™ anti-human CD38 HIT2 FC
Brilliant Violet 605™ anti-human CD38 HIT2 FC
APC/Cyanine7 anti-human CD38 HIT2 FC
Purified anti-human CD38 (Maxpar® Ready) HIT2 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD38 HIT2 FC
PE anti-human CD38 HIT2 FC
Brilliant Violet 510™ anti-human CD38 HIT2 FC
FITC anti-human CD38 HIT2 FC
PE/Dazzle™ 594 anti-human CD38 HIT2 FC
TotalSeq™-A0389 anti-human CD38 HIT2 PG
TotalSeq™-C0389 anti-human CD38 HIT2 PG
APC/Fire™ 750 anti-human CD38 HIT2 FC
TotalSeq™-B0389 anti-human CD38 HIT2 PG
APC/Fire™ 810 anti-human CD38 HIT2 FC
Spark NIR™ 685 anti-human CD38 Antibody HIT2 FC
TotalSeq™-D0389 anti-human CD38 HIT2 PG
PerCP/Cyanine5.5 anti-human CD38 HIT2 FC
APC anti-human CD38 HIT2 FC
APC/Fire™ 750 anti-human CD38 HIT2 FC
PE/Cyanine7 anti-human CD38 HIT2 FC
GMP PE anti-human CD38 HIT2 FC
GMP FITC anti-human CD38 HIT2 FC
Pacific Blue™ anti-human CD38 HIT2 FC
Pacific Blue™ anti-human CD38 HIT2 FC
GMP APC/Fire™ 750 anti-human CD38 HIT2 FC
GMP PE/Dazzle™ 594 anti-human CD38 HIT2 FC
GMP APC anti-human CD38 HIT2 FC
GMP PE/Cyanine7 anti-human CD38 HIT2 FC
Spark Blue™ 515 anti-human CD38 HIT2 FC
GMP Pacific Blue™ anti-human CD38 HIT2 FC
Spark Violet™ 423 anti-human CD38 HIT2 FC
Brilliant Violet 650™ anti-human CD38 HIT2 FC
Spark PLUS B550™ anti-human CD38 HIT2 FC
Go To Top Version: 3    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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