- Clone
- BJ18 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI A034
- Other Names
- Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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338811 | 100 µg | £97 |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Normal human PBL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.
- Product Citations
-
- RRID
-
AB_2562835 (BioLegend Cat. No. 338811)
Antigen Details
- Structure
- Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
- Distribution
-
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
- Function
- Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
- Ligand/Receptor
- Hyaluronan, MIP-1β, fibronectin, collagen
- Cell Type
- Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558. - Gene ID
- 960 View all products for this Gene ID
- UniProt
- View information about CD44 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD44 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD44 | BJ18 | FC,CyTOF®,ICC |
FITC anti-human CD44 | BJ18 | FC |
APC anti-human CD44 | BJ18 | FC |
PE anti-human CD44 | BJ18 | FC |
Brilliant Violet 421™ anti-human CD44 | BJ18 | FC |
Purified anti-human CD44 (Maxpar® Ready) | BJ18 | FC,CyTOF® |
Alexa Fluor® 700 anti-human CD44 | BJ18 | FC |
PE/Dazzle™ 594 anti-human CD44 | BJ18 | FC |
PE/Cyanine7 anti-human CD44 | BJ18 | FC |
APC/Fire™ 750 anti-human CD44 | BJ18 | FC |
PerCP/Cyanine5.5 anti-human CD44 | BJ18 | FC |
Pacific Blue™ anti-human CD44 | BJ18 | FC |
TotalSeq™-A0125 anti-human CD44 | BJ18 | PG |
TotalSeq™-C0125 anti-human CD44 | BJ18 | PG |
Alexa Fluor® 488 anti-human CD44 | BJ18 | FC |
Brilliant Violet 785™ anti-human CD44 | BJ18 | FC |
TotalSeq™-B0125 anti-human CD44 Antibody | BJ18 | PG |
TotalSeq™-D0125 anti-human CD44 | BJ18 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD44
MDA-MB-231 cells were stained with anti-CD44 (clone BJ18), f... -
FITC anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 FITC -
APC anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 APC -
PE anti-human CD44
Human peripheral blood lymphocytes stained with BJ18 PE Pre-lysed human blood leukocytes were stained with CD44 (clo... -
Brilliant Violet 421™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
Purified anti-human CD44 (Maxpar® Ready)
Human PBMCs stained with 166Er-anti-CD44 (BJ18) and 158Gd-an... -
Alexa Fluor® 700 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PE/Dazzle™ 594 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PE/Cyanine7 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
APC/Fire™ 750 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
PerCP/Cyanine5.5 anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
Pacific Blue™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
TotalSeq™-A0125 anti-human CD44
-
TotalSeq™-C0125 anti-human CD44
-
Alexa Fluor® 488 anti-human CD44
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 785™ anti-human CD44
Human peripheral blood lymphocytes were stained with CD44 (c... -
TotalSeq™-B0125 anti-human CD44 Antibody
-
TotalSeq™-D0125 anti-human CD44
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