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Purified anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_HI100_Purified_062707
Human peripheral blood platelets stained with purified HI100, followed by anti-mouse IgGs FITC
  • A_HI100_Purified_062707
    Human peripheral blood platelets stained with purified HI100, followed by anti-mouse IgGs FITC
  • B_HI100_PURE_CD45RA_Antibody_SB_111423
    SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-CD45RA (clone HI100, yellow) on formalin-fixed paraffin-embedded human pancreatic carcinoma tissue at 0.5 µg/mL. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
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304102 100 µg £31
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IHC-F, IHC-P, PG - Reported in the literature, not verified in house
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  2. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  3. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  4. Dao T, et al. 2022. JCI Insight. 7: . PubMed
  5. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  6. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  7. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  8. Søgaard O, et al. 2015. PLoS One. 11: 1005142. PubMed
  9. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  10. Sullivan KD, et al. 2021. Cell Reports. 36(7):109527. PubMed
  11. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  12. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  13. Damond N, et al. 2019. Cell Metab. 29:755. PubMed
  14. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  15. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  16. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  17. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  18. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  19. Taylor JG, et al. 2022. Haematologica. :. PubMed
  20. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  21. Hsiao F, et al. 2020. PLoS Pathog. 16:e1008450. PubMed
  22. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  23. Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed
  24. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  25. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  26. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  27. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  28. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  29. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  30. Michlmayr D, et al. 2020. Cell Reports. 31(4):107569. PubMed
  31. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  32. Galbraith MD, et al. 2021. eLife. 10:00. PubMed
  33. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  34. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  35. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  36. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  37. Lückel C, et al. 2019. Nat Commun. 4.390277778. PubMed
  38. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  39. Wu Y, et al. 2007. Infect Immun . 178:2802. PubMed
  40. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  41. NULL, et al. 2022. Cell. 185:916. PubMed
  42. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  43. Lazarski CA, et al. 2020. Cytotherapy. . PubMed
  44. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  45. Nicolet BP, et al. 2017. J Immunol. 198:962. PubMed
  46. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  47. Guerau-de-Arellano M, et al. 2011. Brain. 134:3578. PubMed
  48. Oda H, et al. 2019. Front Immunol. 10:479. PubMed
  49. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  50. Wadley AJ, et al. 2020. Brain Behav Immun Health. 3:100049. PubMed
  51. Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed
  52. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  53. Martin E, et al. 2020. JCI Insight. :5. PubMed
  54. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  55. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  56. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_314406 (BioLegend Cat. No. 304102)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD45RA Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45RA HI100 FC
Biotin anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
PE anti-human CD45RA HI100 FC,SB
PE/Cyanine5 anti-human CD45RA HI100 FC
Purified anti-human CD45RA HI100 FC,CyTOF®,ICC,IHC-P,IHC-F,SB
Alexa Fluor® 488 anti-human CD45RA HI100 FC
Alexa Fluor® 647 anti-human CD45RA HI100 FC,IHC-P
Pacific Blue™ anti-human CD45RA HI100 FC
Alexa Fluor® 700 anti-human CD45RA HI100 FC
PerCP/Cyanine5.5 anti-human CD45RA HI100 FC,SB
PE/Cyanine7 anti-human CD45RA HI100 FC
APC/Cyanine7 anti-human CD45RA HI100 FC
Brilliant Violet 421™ anti-human CD45RA HI100 FC,IHC-P
Brilliant Violet 570™ anti-human CD45RA HI100 FC
Brilliant Violet 605™ anti-human CD45RA HI100 FC
Brilliant Violet 650™ anti-human CD45RA HI100 FC
Brilliant Violet 711™ anti-human CD45RA HI100 FC
Brilliant Violet 785™ anti-human CD45RA HI100 FC
Brilliant Violet 510™ anti-human CD45RA HI100 FC
Purified anti-human CD45RA (Maxpar® Ready) HI100 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
PerCP anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
TotalSeq™-A0063 anti-human CD45RA HI100 PG
Alexa Fluor® 594 anti-human CD45RA HI100 IHC-P
TotalSeq™-B0063 anti-human CD45RA HI100 PG
TotalSeq™-C0063 anti-human CD45RA HI100 PG
Brilliant Violet 750™ anti-human CD45RA HI100 FC
Spark NIR™ 685 anti-human CD45RA HI100 FC
APC anti-human CD45RA HI100 FC
PE/Fire™ 640 anti-human CD45RA HI100 FC
PE/Fire™ 700 anti-human CD45RA Antibody HI100 FC
Spark YG™ 581 anti-human CD45RA HI100 FC
TotalSeq™-D0063 anti-human CD45RA HI100 PG
Spark Violet™ 423 anti-human CD45RA HI100 FC,IHC-P
GMP FITC anti-human CD45RA HI100 FC
PE/Cyanine7 anti-human CD45RA HI100 FC
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark UV™ 387 anti-human CD45RA HI100 FC
GMP APC anti-human CD45RA HI100 FC
TotalSeq™-Bn0063 anti-human CD45RA HI100 SB
Spark Blue™ 550 anti-human CD45RA HI100 FC
GMP PE/Dazzle™ 594 anti-human CD45RA HI100 FC
GMP APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark PLUS UV395™ anti-human CD45RA HI100 FC
Spark Red™ 718 anti-human CD45RA HI100 FC
APC/Fire™ 810 anti-human CD45RA HI100 FC
PE/Fire™ 810 anti-human CD45RA HI100 FC
Spark Blue™ 574 anti-human CD45RA (Flexi-Fluor™) HI100 FC
Go To Top Version: 4    Revision Date: 11/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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